Contact Guidance by Microstructured Gelatin Hydrogels for Prospective Tissue Engineering Applications

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Tadsen M, Friedrich RP, Riedel S, Alexiou C, Mayr SG
Zeitschrift: ACS Applied Materials and Interfaces
Jahr der Veröffentlichung: 2019
Band: 11
Heftnummer: 7
Seitenbereich: 7450-7458
ISSN: 1944-8244


Abstract

Design of functionalized biomimetic scaffolds is one of the key approaches for regenerative medicine and other biomedical applications. Development of engineered tissue should optimize organization and function of cells and tissue in vitro as well as in vivo. Surface topography is one factor controlling cellular behavior and tissue development. By topographical patterning of biocompatible materials, highly functionalized scaffolds can be developed. Gelatin is hereby a promising candidate due to its biocompatibility and biodegradability. It is low in cost and easy to handle, enabling a variety of applications in science and medicine. However, for biomedical applications at physiological conditions, gelatin has to be additionally stabilized since its gel-sol-transition temperature lies beneath the human body temperature. This is realized by a reagent-free cross-linking technique utilizing electron beam treatment. By topographical patterning, gelatin can be functionalized toward scaffolds for cell cultivation and tissue development. Thereby, customized patterns are transferred onto gelatin hydrogels via molds. Thermal stabilization of gelatin is then achieved by electron-induced cross-linking. In this study, we investigate the influence of gelatin concentration and irradiation dose on pattern transfer, long-term stability of topographically patterned gelatin hydrogels, and their impact on the cellular behavior of human umbilical vein endothelial cells as well as normal human dermal fibroblasts. We will show that contact guidance occurs for both cell types due to a concrete stripe pattern. In addition, the presented studies show a high degree of cytocompatibility, indicating a high potential of topographically patterned gelatin hydrogels as tissue development scaffold for prospective biomedical applications.


FAU-Autoren / FAU-Herausgeber

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin


Autor(en) der externen Einrichtung(en)
Leibniz-Institute für Oberflächenmodifizierung e. V. (IOM) / Leibniz Institute of Surface Modification


Zitierweisen

APA:
Tadsen, M., Friedrich, R.P., Riedel, S., Alexiou, C., & Mayr, S.G. (2019). Contact Guidance by Microstructured Gelatin Hydrogels for Prospective Tissue Engineering Applications. ACS Applied Materials and Interfaces, 11(7), 7450-7458. https://dx.doi.org/10.1021/acsami.8b21638

MLA:
Tadsen, Meike, et al. "Contact Guidance by Microstructured Gelatin Hydrogels for Prospective Tissue Engineering Applications." ACS Applied Materials and Interfaces 11.7 (2019): 7450-7458.

BibTeX: 

Zuletzt aktualisiert 2019-20-03 um 11:53