Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC
Schierer S, Ostalecki C, Zinser E, Lamprecht R, Plosnita B, Stich L, Dörrie J, Lutz MB, Schuler G, Baur A (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 1
Journal Issue: 6
Abstract
During inflammation, murine and human monocytes can develop into dendritic cells (DC), but this process is not entirely understood. Here, we demonstrate that extracellular vesicles (EV) secreted by mature human DC (maDC) differentiate peripheral monocytes into immature DC, expressing a unique marker pattern, including 6-sulfo LacNAc (slan), Zbtb46, CD64, and CD14. While EV from both maDC and immature DC differentiated monocytes similar to GM-CSF/IL-4 stimulation, only maDC-EV produced precursors, which upon maturation stimulus developed into T-cell-activating and IL-12p70-secreting maDC. Mechanistically, maDC-EV induced cell signaling through GM-CSF, which was abundant in EV as were IL-4 and other cytokines and chemokines. When injected into the mouse skin, murine maDC-EV attracted immune cells including monocytes that developed activation markers typical for inflammatory cells. Skin-injected EV also reached lymph nodes, causing a similar immune cell infiltration. We conclude that DC-derived EV likely serve to perpetuate an immune reaction and may contribute to chronic inflammation.
Authors with CRIS profile
Ricarda Lamprecht-Bailer
Medizinische Fakultät
Jan Dörrie
Department of Dermatology
Gerold Schuler
Lehrstuhl für Haut- und Geschlechtskrankheiten
Involved external institutions
Austria (AT)
Germany (DE)How to cite
APA:
Schierer, S., Ostalecki, C., Zinser, E., Lamprecht, R., Plosnita, B., Stich, L.,... Baur, A. (2018). Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC. Life Science Alliance, 1(6). https://doi.org/10.26508/lsa.201800093
MLA:
Schierer, Stefan, et al. "Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC." Life Science Alliance 1.6 (2018).
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