Unimpaired Responses to Vaccination With Protein Antigen Plus Adjuvant in Mice With Kit-Independent Mast Cell Deficiency

Schubert N, Lisenko K, Auerbach C, Weitzmann A, Ghouse SM, Muhandes L, Haase C, Haering T, Schulze L, Vöhringer D, Gunzer F, Mueller W, Feyerabend TB, Rodewald HR, Dudeck A, Roers A (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Frontiers in Immunology Frontiers Research Foundation / Frontiers Media

Book Volume: 9

DOI: 10.3389/fimmu.2018.01870

Abstract

Innate inflammatory responses are crucial for induction and regulation of T cell and antibody responses. Mast cell (MC)-deficient Kit mutant mice showed impaired adaptive immunity, suggesting that MCs provide essential adjuvant activities, and pharmacological MC activation was proposed as a new adjuvant principle. However, the Kit mutations result in complex alterations of the immune system in addition to MC deficiency. We revisited the role of MCs in vaccination responses using Mcpt5-Cre R26DTA/DTA and Cpa3Cre/+ mice that lack connective tissue MCs or all MCs, respectively, but feature an otherwise normal immune system. These animals showed no impairment of T and B cell responses to intradermal vaccination with protein antigen plus complete Freund's adjuvant. Moreover, we demonstrate that the adjuvant effects of the MC secretagogue c48/80 in intradermal or mucosal immunization are independent of the presence of MCs. We hence find no evidence for a regulation by MCs of adaptive immune responses to protein antigens. The finding that immunological MC functions differ from those suggested by experiments in Kit mutants, emphasizes the importance of rigorous tests in Kit-independent MC-deficiency models.

Authors with CRIS profile

David Vöhringer Professur für Infektionsabwehr und Toleranz

Involved external institutions

Technische Universität Dresden DE Germany (DE) Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE) University of Manchester GB United Kingdom (GB)

How to cite

APA:

Schubert, N., Lisenko, K., Auerbach, C., Weitzmann, A., Ghouse, S.M., Muhandes, L.,... Roers, A. (2018). Unimpaired Responses to Vaccination With Protein Antigen Plus Adjuvant in Mice With Kit-Independent Mast Cell Deficiency. Frontiers in Immunology, 9. https://dx.doi.org/10.3389/fimmu.2018.01870

MLA:

Schubert, Nadja, et al. "Unimpaired Responses to Vaccination With Protein Antigen Plus Adjuvant in Mice With Kit-Independent Mast Cell Deficiency." Frontiers in Immunology 9 (2018).

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