Comparative analysis of nanosystems' effects on human endothelial and monocytic cell functions

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Matuszak J, Doerfler P, Lyer S, Unterweger H, Juenet M, Chauvierre C, Alaarg A, Franke D, Almer G, Texier I, Metselaar JM, Prassl R, Alexiou C, Mangge H, Letourneur D, Cicha I
Zeitschrift: Nanotoxicology
Jahr der Veröffentlichung: 2018
Band: 12
Heftnummer: 9
Seitenbereich: 957-974
ISSN: 1743-5390


The objective of our work was to investigate the effects of different types of nanoparticles on endothelial (HUVEC) and monocytic cell functions. We prepared and tested 14 different nanosystems comprising liposomes, lipid nanoparticles, polymer, and iron oxide nanoparticles. Some of the tested nanosystems contained targeting, therapeutic, or contrast agent(s). The effect of particles (0-400 mu g/mL) on endothelial-monocytic cell interactions in response to TNF-alpha was investigated using an arterial bifurcation model and dynamic monocyte adhesion assay. Spontaneous HUVEC migration (0-100 mu g/mL nanoparticles) and chemotaxis of monocytic cells towards MCP-1 in presence of particles (0-400 mu g/mL) were determined using a barrier assay and a modified Boyden chamber assay, respectively. Lipid nanoparticles dose-dependently reduced monocytic cell chemotaxis and adhesion to activated HUVECs. Liposomal nanoparticles had little effect on cell migration, but one formulation induced monocytic cell recruitment by HUVECs under non-uniform shear stress by about 50%. Fucoidan-coated polymer nanoparticles (25-50 mu g/mL) inhibited HUVEC migration and monocytic cell chemotaxis, and had a suppressive effect on monocytic cell recruitment under non-uniform shear stress. No significant effects of iron oxide nanoparticles on monocytic cell recruitment were observed except lauric acid and human albumin-coated particles which increased endothelial-monocytic interactions by 60-70%. Some of the iron oxide nanoparticles inhibited HUVEC migration and monocytic cell chemotaxis. These nanoparticle-induced effects are of importance for vascular cell biology and function and must be considered before the potential clinical use of some of the analyzed nanosystems in cardiovascular applications.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Cicha, Iwona Prof. Dr.
Medizinische Fakultät
Lyer, Stefan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Matuszak, Jasmin
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie

Einrichtungen weiterer Autorinnen und Autoren

nanoPET Pharma GmbH
University of Grenoble Alpes (UGA) / Université de Grenoble
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
University of Twente


Matuszak, J., Doerfler, P., Lyer, S., Unterweger, H., Juenet, M., Chauvierre, C.,... Cicha, I. (2018). Comparative analysis of nanosystems' effects on human endothelial and monocytic cell functions. Nanotoxicology, 12(9), 957-974.

Matuszak, Jasmin, et al. "Comparative analysis of nanosystems' effects on human endothelial and monocytic cell functions." Nanotoxicology 12.9 (2018): 957-974.


Zuletzt aktualisiert 2019-08-03 um 12:38