Transcriptome-wide analysis of filarial extract-primed human monocytes reveal changes in LPS-induced PTX3 expression levels

Journal article


Publication Details

Author(s): Buerfent BC, Gölz L, Hofmann A, Ruehl H, Stamminger W, Fricker N, Hess T, Oldenburg J, Noethen MM, Schumacher J, Huebner MP, Hoerauf A
Journal: Scientific Reports
Publication year: 2019
Volume: 9
ISSN: 2045-2322


Abstract

Filarial nematodes modulate immune responses in their host to enable their survival and mediate protective effects against autoimmunity and allergies. In this study, we examined the immunomodulatory capacity of extracts from the human pathogenic filaria Brugia malayi (BmA) on human monocyte responses in a transcriptome-wide manner to identify associated pathways and diseases. As previous transcriptome studies often observed quiescent responses of innate cells to filariae, the potential of BmA to alter LPS driven responses was investigated by analyzing >47.000 transcripts of monocytes from healthy male volunteers stimulated with BmA, Escherichia coli LPS or a sequential stimulation of both. In comparison to-2200 differentially expressed genes in LPS-only stimulated monocytes, only a limited number of differentially expressed genes were identified upon BmA priming before LPS re-stimulation with only PTX34 down arrow reaching statistical significance after correcting for multiple testing. Nominal significant differences were reached for meta llothioneins up arrow, MMP9 up arrow, CXCL5/ENA-78 up arrow, CXCL6/GCP-2 up arrow, TNFRSF21 down arrow, and CCL20/MIP3 alpha down arrow and were confirmed by qPCR or ELISA. Flow cytometric analysis of activation markers revealed a reduced LPS-induced expression of HLA-DR and CD86 on BmA-primed monocytes as well as a reduced apoptosis of BmA-stimulated monocytes. While our experimental design does not allow a stringent extrapolation of our results to the development of filarial pathology, several genes that were identified in BmA-primed monocytes had previously been associated with filarial pathology, supporting the need for further research.


FAU Authors / FAU Editors

Gölz, Lina Prof. Dr.
Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie


External institutions with authors

Rheinische Friedrich-Wilhelms-Universität Bonn
Universitätsklinikum Bonn


How to cite

APA:
Buerfent, B.C., Gölz, L., Hofmann, A., Ruehl, H., Stamminger, W., Fricker, N.,... Hoerauf, A. (2019). Transcriptome-wide analysis of filarial extract-primed human monocytes reveal changes in LPS-induced PTX3 expression levels. Scientific Reports, 9. https://dx.doi.org/10.1038/s41598-019-38985-x

MLA:
Buerfent, B. C., et al. "Transcriptome-wide analysis of filarial extract-primed human monocytes reveal changes in LPS-induced PTX3 expression levels." Scientific Reports 9 (2019).

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Last updated on 2019-12-03 at 08:23