Phosphorylation Modulates the Subcellular Localization of SOX11

Balta EA, Wittmann MT, Jung M, Sock E, Häberle B, Heim B, Von Zweydorf F, Heppt J, von Wittgenstein J, Gloeckner CJ, Lie DC (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Frontiers in Molecular Neuroscience Frontiers Research Foundation / Frontiers Media

Book Volume: 11

DOI: 10.3389/fnmol.2018.00211

Abstract

SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11's transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.

Authors with CRIS profile

Elli-Anna Balta Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung Marie-Theres Wittmann Lehrstuhl für Humangenetik Matthias Jung Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung Elisabeth Sock Lehrstuhl für Biochemie und Pathobiochemie Benjamin Häberle Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung Jana Heppt Interdisziplinäres Zentrum für Klinische Forschung IZKF-Förderlinien Julia von Wittgenstein Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung Dieter Chichung Lie Institut für Biochemie

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung

Involved external institutions

Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) DE Germany (DE) Eberhard Karls Universität Tübingen DE Germany (DE)

How to cite

APA:

Balta, E.-A., Wittmann, M.-T., Jung, M., Sock, E., Häberle, B., Heim, B.,... Lie, D.C. (2018). Phosphorylation Modulates the Subcellular Localization of SOX11. Frontiers in Molecular Neuroscience, 11. https://doi.org/10.3389/fnmol.2018.00211

MLA:

Balta, Elli-Anna, et al. "Phosphorylation Modulates the Subcellular Localization of SOX11." Frontiers in Molecular Neuroscience 11 (2018).

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