Probe-based confocal laser endomicroscopy in detecting malignant lesions of vocal folds.

Goncalves M, Aubreville M, Mueller SK, Sievert M, Maier A, Iro H, Bohr C (2019)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2019

Journal

Acta Otorhinolaryngologica Italica Pacini Editore SpA

DOI: 10.14639/0392-100X-2121

Open Access Link: https://www.actaitalica.it/article/view/117/197

Abstract

Probe-based confocal laser endomicroscopy (CLE) is an innovative technique for real-time, non-invasive analysis of the surface epithelium. While being successfully used for diagnosis by experts, this method has not yet been established in clinical routine, partly due to the lack of standards and criteria for classifying various lesions. Our aim was to determine the diagnostic value and inter-rater reliability of CLE in detecting malignant lesions of the vocal cords. 58 video sequences were extracted from the probe-based CLE (GastroFlex probe with a Cellvizio® laser system) examinations of 3 patients with squamous cell carcinomas and 4 patients with benign alterations of the vocal folds. Two ENT surgeons, who were blinded to the histological result, were asked to identify the sequences representing a carcinoma. We showed an accuracy, sensitivity, specificity, PPV and NPV of 91.38-96.55%, 100%, 87.8-95.2%, 77.27-89.47% and 100%, respectively, with an inter-rater reliability of k = 0.89 ("almost perfect agreement"). Probe-based CLE is a promising method for diagnosis and assessment of vocal fold lesions in vivo. Our results suggest that, with adequate training, the diagnostic value of this technique can be improved and potentially provide important information during oncological surgery.

Authors with CRIS profile

Miguel Goncalves Department of Otorhinolaryngology – Head and Neck Surgery Marc Aubreville Lehrstuhl für Informatik 14 (Bild- und Sprachverarbeitung) Sarina Müller-Hübner Department of Otorhinolaryngology – Head and Neck Surgery Matti Sievert Department of Otorhinolaryngology – Head and Neck Surgery Andreas Maier Lehrstuhl für Informatik 5 (Mustererkennung) Heinrich Iro Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde

Related research project(s)

Automatic classification and image analysis of confocal laser endomicroscopy images Oct. 1, 2014 - Oct. 1, 2017

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE)

How to cite

APA:

Goncalves, M., Aubreville, M., Mueller, S.K., Sievert, M., Maier, A., Iro, H., & Bohr, C. (2019). Probe-based confocal laser endomicroscopy in detecting malignant lesions of vocal folds. Acta Otorhinolaryngologica Italica. https://dx.doi.org/10.14639/0392-100X-2121

MLA:

Goncalves, Miguel, et al. "Probe-based confocal laser endomicroscopy in detecting malignant lesions of vocal folds." Acta Otorhinolaryngologica Italica (2019).

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