Transcriptome, proteome and draft genome of Euglena gracilis

Journal article


Publication Details

Author(s): Ebenezer TE, Zoltner M, Burrell A, Nenarokova A, Vanclova AMGN, Prasad B, Soukal P, Santana-Molina C, O'Neill E, Nankissoor NN, Vadakedath N, Daiker V, Obado S, Silva-Pereira S, Jackson AP, Devos DP, Lukes J, Lebert M, Vaughan S, Hampl V, Carrington M, Ginger ML, Dacks JB, Kelly S, Field MC
Journal: BMC Biology
Publication year: 2019
Volume: 17
ISSN: 1741-7007


Abstract

BackgroundPhotosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gracilis has been a popular model organism and of considerable biotechnological interest, but the absence of a gene catalogue has hampered both basic research and translational efforts.ResultsWe report a detailed transcriptome and partial genome for E. gracilis Z1. The nuclear genome is estimated to be around 500Mb in size, and the transcriptome encodes over 36,000 proteins and the genome possesses less than 1% coding sequence. Annotation of coding sequences indicates a highly sophisticated endomembrane system, RNA processing mechanisms and nuclear genome contributions from several photosynthetic lineages. Multiple gene families, including likely signal transduction components, have been massively expanded. Alterations in protein abundance are controlled post-transcriptionally between light and dark conditions, surprisingly similar to trypanosomatids.ConclusionsOur data provide evidence that a range of photosynthetic eukaryotes contributed to the Euglena nuclear genome, evidence in support of the shopping bag' hypothesis for plastid acquisition. We also suggest that euglenids possess unique regulatory mechanisms for achieving extreme adaptability, through mechanisms of paralog expansion and gene acquisition.


FAU Authors / FAU Editors

Daiker, Viktor Dr.
Lehrstuhl für Zellbiologie
Lebert, Michael PD Dr.
Lehrstuhl für Zellbiologie
Prasad, Binod Dr.-Ing.
Lehrstuhl für Zellbiologie


External institutions
Oxford Brookes University
Rockefeller University
The Czech Academy of Sciences
The University of Liverpool
Universidad Pablo de Olavide of Seville
University of Alberta
University of Cambridge
University of Dundee
University of Huddersfield
University of Oxford
Univerzita Karlova v Praze / Charles University in Prague


How to cite

APA:
Ebenezer, T.E., Zoltner, M., Burrell, A., Nenarokova, A., Vanclova, A.M.G.N., Prasad, B.,... Field, M.C. (2019). Transcriptome, proteome and draft genome of Euglena gracilis. BMC Biology, 17. https://dx.doi.org/10.1186/s12915-019-0626-8

MLA:
Ebenezer, Thankgod E., et al. "Transcriptome, proteome and draft genome of Euglena gracilis." BMC Biology 17 (2019).

BibTeX: 

Last updated on 2019-20-03 at 16:38