ADAR1 Is Required for Dendritic Cell Subset Homeostasis and Alveolar Macrophage Function

Journal article


Publication Details

Author(s): Baal N, Cunningham S, Obermann HL, Thomas J, Lippitsch A, Dietert K, Gruber AD, Kaufmann A, Michel G, Nist A, Stiewe T, Rupp O, Goesmann A, Zukunft S, Fleming I, Bein G, Lohmeyer J, Bauer S, Hackstein H
Journal: Journal of Immunology
Publication year: 2019
Volume: 202
Journal issue: 4
ISSN: 0022-1767


Abstract

RNA editing by adenosine deaminases acting on dsRNA (ADAR) has become of increasing medical relevance, particularly because aberrant ADAR1 activity has been associated with autoimmunity and malignancies. However, the role of ADAR1 in dendritic cells (DC), representing critical professional APCs, is unknown. We have established conditional murine CD11c Cre-mediated ADAR1 gene ablation, which did not induce general apoptosis in CD11c(+) cells but instead manifests in cell type-specific effects in DC subpopulations. Bone marrow-derived DC subset analysis revealed an incapacity to differentiate CD103 DC+ in both bulk bone marrow and purified pre-DC lineage progenitor assays. ADAR1 deficiency further resulted in a preferential systemic loss of CD8(+)/CD103(+) DCs, revealing critical dependency on ADAR1, whereas other DC subpopulations were moderately affected or unaffected. Additionally, alveolar macrophages were depleted and dysfunctional, resembling pulmonary alveolar proteinosis. These results reveal an unrecognized role of ADAR1 in DC subset homeostasis and unveils the cell type-specific effects of RNA editing.


FAU Authors / FAU Editors

Hackstein, Holger Prof. Dr. med.
Professur für Transfusionsmedizin und Cell-Engineering


External institutions with authors

Freie Universität Berlin
Goethe-Universität Frankfurt am Main
Justus-Liebig-Universität Gießen
Philipps-Universität Marburg


How to cite

APA:
Baal, N., Cunningham, S., Obermann, H.-L., Thomas, J., Lippitsch, A., Dietert, K.,... Hackstein, H. (2019). ADAR1 Is Required for Dendritic Cell Subset Homeostasis and Alveolar Macrophage Function. Journal of Immunology, 202(4). https://dx.doi.org/10.4049/jimmunol.1800269

MLA:
Baal, Nelli, et al. "ADAR1 Is Required for Dendritic Cell Subset Homeostasis and Alveolar Macrophage Function." Journal of Immunology 202.4 (2019).

BibTeX: 

Last updated on 2019-22-02 at 11:23