Hair Follicle Stem Cell Isolation and Expansion

Journal article

Publication Details

Author(s): Call M, Meyer EA, Kao WW, Kruse F, Schlötzer-Schrehardt U
Journal: Bio-protocol
Publication year: 2018
Volume: 8
Journal issue: 10
ISSN: 2331-8325


Stem cells are widely used for numerous clinical applications including limbal stem cell deficiency. Stem cell derived from the bulge region of the hair follicle have the ability to differentiate into a variety of cell types including interfollicular epidermis, hair follicle structures, sebaceous glands and corneal epithelial cells when provided the appropriate cues. Hair follicle stem cells are being studied as a valuable source of autologous stem cells to treat disease. The protocol described below details the isolation and expansion of these cells for eventual clinical application. We used a dual-reporter mouse model to visualize both isolation and eventual differentiation of these cells in a limbal stem cell-deficient mouse model.

FAU Authors / FAU Editors

Kruse, Friedrich Prof. Dr.
Lehrstuhl für Augenheilkunde
Schlötzer-Schrehardt, Ursula apl. Prof. Dr.
Medizinische Fakultät

External institutions with authors

University of Cincinnati

How to cite

Call, M., Meyer, E.A., Kao, W.W., Kruse, F., & Schlötzer-Schrehardt, U. (2018). Hair Follicle Stem Cell Isolation and Expansion. Bio-protocol, 8(10).

Call, Mindy, et al. "Hair Follicle Stem Cell Isolation and Expansion." Bio-protocol 8.10 (2018).


Last updated on 2019-25-02 at 10:08