Hair Follicle Stem Cell Isolation and Expansion

Call M, Meyer EA, Kao WW, Kruse F, Schlötzer-Schrehardt U (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Bio-protocol Bio-Protocol

Book Volume: 8

Article Number: e2848

Journal Issue: 10

DOI: 10.21769/BioProtoc.2848

Abstract

Stem cells are widely used for numerous clinical applications including limbal stem cell deficiency. Stem cell derived from the bulge region of the hair follicle have the ability to differentiate into a variety of cell types including interfollicular epidermis, hair follicle structures, sebaceous glands and corneal epithelial cells when provided the appropriate cues. Hair follicle stem cells are being studied as a valuable source of autologous stem cells to treat disease. The protocol described below details the isolation and expansion of these cells for eventual clinical application. We used a dual-reporter mouse model to visualize both isolation and eventual differentiation of these cells in a limbal stem cell-deficient mouse model.

Authors with CRIS profile

Friedrich Kruse Lehrstuhl für Augenheilkunde Ursula Schlötzer-Schrehardt Medizinische Fakultät

Involved external institutions

University of Cincinnati US United States (USA) (US)

How to cite

APA:

Call, M., Meyer, E.A., Kao, W.W., Kruse, F., & Schlötzer-Schrehardt, U. (2018). Hair Follicle Stem Cell Isolation and Expansion. Bio-protocol, 8(10). https://doi.org/10.21769/BioProtoc.2848

MLA:

Call, Mindy, et al. "Hair Follicle Stem Cell Isolation and Expansion." Bio-protocol 8.10 (2018).

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