Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier
Call M, Meyer EA, Kao WW, Kruse F, Schlötzer-Schrehardt U (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 8
Article Number: e2849
Journal Issue: 10
Abstract
The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice expressing a dual reporter gene under the control of the Tet-on system and the keratin 12 promoter (Meyer-Blazejewska et al., 2011). A clonal growth assay was performed to enrich for stem cells. Once holoclones formed they were transferred onto a fibrin carrier and cultivated to obtain a confluent epithelial cell layer. Limbal stem cell deficient (LSCD) mice were used as the transplant recipient in order to test for successful grafting and eventual differentiation into a corneal epithelial phenotype.
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APA:
Call, M., Meyer, E.A., Kao, W.W., Kruse, F., & Schlötzer-Schrehardt, U. (2018). Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier. Bio-protocol, 8(10). https://dx.doi.org/10.21769/BioProtoc.2849
MLA:
Call, Mindy, et al. "Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier." Bio-protocol 8.10 (2018).
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