Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast
Strelnikova N, Sauter N, Guizar-Sicairos M, Gollner M, Diaz A, Delivani P, Chacon M, Tolic IM, Zaburdaev V, Pfohl T (2017)
Publication Status: Published
Publication Type: Journal article
Publication year: 2017
Journal
Publisher: NATURE PUBLISHING GROUP
Book Volume: 7
DOI: 10.1038/s41598-017-13175-9
Abstract
Seeing physiological processes at the nanoscale in living organisms without labeling is an ultimate goal in life sciences. Using X-ray ptychography, we explored in situ the dynamics of unstained, living fission yeast Schizosaccharomyces pombe cells in natural, aqueous environment at the nanoscale. In contrast to previous X-ray imaging studies on biological matter, in this work the eukaryotic cells were alive even after several ptychographic X-ray scans, which allowed us to visualize the chromatin motion as well as the autophagic cell death induced by the ionizing radiation. The accumulated radiation of the sequential scans allowed for the determination of a characteristic dose of autophagic vacuole formation and the lethal dose for fission yeast. The presented results demonstrate a practical method that opens another way of looking at living biological specimens and processes in a time-resolved label-free setting.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Universität Basel
Switzerland (CH)
Paul Scherrer Institute (PSI)
Switzerland (CH)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
Germany (DE)
Universität Basel
Switzerland (CH)
Paul Scherrer Institute (PSI)
Switzerland (CH)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
How to cite
APA:
Strelnikova, N., Sauter, N., Guizar-Sicairos, M., Gollner, M., Diaz, A., Delivani, P.,... Pfohl, T. (2017). Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast. Scientific Reports, 7. https://doi.org/10.1038/s41598-017-13175-9
MLA:
Strelnikova, Natalja, et al. "Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast." Scientific Reports 7 (2017).
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