A tunable refractive index matching medium for live imaging cells, tissues and model organisms
Boothe T, Hilbert L, Heide M, Berninger L, Huttner WB, Zaburdaev V, Vastenhouw NL, Myers EW, Drechsel DN, Rink JC (2017)
Publication Status: Published
Publication Type: Journal article
Publication year: 2017
Journal
Publisher: ELIFE SCIENCES PUBLICATIONS LTD
Book Volume: 6
DOI: 10.7554/eLife.27240
Abstract
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
Germany (DE)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
How to cite
APA:
Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V.,... Rink, J.C. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLife, 6. https://dx.doi.org/10.7554/eLife.27240
MLA:
Boothe, Tobias, et al. "A tunable refractive index matching medium for live imaging cells, tissues and model organisms." eLife 6 (2017).
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