A tunable refractive index matching medium for live imaging cells, tissues and model organisms

Journal article


Publication Details

Author(s): Boothe T, Hilbert L, Heide M, Berninger L, Huttner WB, Zaburdaev V, Vastenhouw NL, Myers EW, Drechsel DN, Rink JC
Journal: eLife
Publisher: ELIFE SCIENCES PUBLICATIONS LTD
Publication year: 2017
Volume: 6
ISSN: 2050-084X


Abstract

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.


Additional Organisation
Lehrstuhl für Mathematik in den Lebenswissenschaften


External institutions with authors

Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems


How to cite

APA:
Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V.,... Rink, J.C. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLife, 6. https://dx.doi.org/10.7554/eLife.27240

MLA:
Boothe, Tobias, et al. "A tunable refractive index matching medium for live imaging cells, tissues and model organisms." eLife 6 (2017).

BibTeX: 

Last updated on 2019-12-02 at 10:38