Plasma gelsolin promotes re-epithelialization

Journal article


Publication Details

Author(s): Wittmann J, Dieckow J, Schröder H, Hampel U, Garreis F, Jacobi C, Milczarek A, Hsieh KL, Pulli B, Chen JW, Hoogeboom S, Bräuer L, Paulsen F, Schob S, Schicht M
Journal: Scientific Reports
Publication year: 2018
Volume: 8
Journal issue: 1
ISSN: 2045-2322


Abstract

Woundhealing disorders characterized by impaired or delayed re-epithelialization are a serious medical problem that is painful and difficult to treat. Gelsolin (GSN), a known actin modulator, supports epithelial cell regeneration and apoptosis. The aim of this study was to estimate the potential of recombinant gelsolin (rhu-pGSN) for ocular surface regeneration to establish a novel therapy for delayed or complicated wound healing. We analyzed the influence of gelsolin on cell proliferation and wound healing in vitro, in vivo/ex vivo and by gene knockdown. Gelsolin is expressed in all tested tissues of the ocular system as shown by molecular analysis. The concentration of GSN is significantly increased in tear fluid samples of patients with dry eye disease. rhu-pGSN induces cell proliferation and faster wound healing in vitro as well as in vivo/ex vivo. TGF-β dependent transcription of SMA is significantly decreased after GSN gene knockdown. Gelsolin is an inherent protein of the ocular system and is secreted into the tear fluid. Our results show a positive effect on corneal cell proliferation and wound healing. Furthermore, GSN regulates the synthesis of SMA in myofibroblasts, which establishes GSN as a key protein of TGF-β dependent cell differentiation.


FAU Authors / FAU Editors

Bräuer, Lars Prof. Dr.
Professur für Oberflächenaktive Proteine
Garreis, Fabian PD Dr.
Lehrstuhl für Anatomie II
Hampel, Ulrike PD Dr.
Lehrstuhl für Anatomie II
Jacobi, Christina PD Dr.
Medizinische Fakultät
Milczarek, Aleksandra
Lehrstuhl für Anatomie II
Paulsen, Friedrich Prof. Dr.
Lehrstuhl für Anatomie II
Schicht, Martin Dr.
Lehrstuhl für Anatomie II
Schröder, Henrik
Professur für Oberflächenaktive Proteine
Wittmann, Johannes
Lehrstuhl für Anatomie II


External institutions with authors

Fraunhofer-Institut für Integrierte Schaltungen (IIS)
Massachusetts General Hospital
Taipei Medical University Hospital (TMUH) / 臺北醫學大學附設醫院
Universität Leipzig


How to cite

APA:
Wittmann, J., Dieckow, J., Schröder, H., Hampel, U., Garreis, F., Jacobi, C.,... Schicht, M. (2018). Plasma gelsolin promotes re-epithelialization. Scientific Reports, 8(1). https://dx.doi.org/10.1038/s41598-018-31441-2

MLA:
Wittmann, Johannes, et al. "Plasma gelsolin promotes re-epithelialization." Scientific Reports 8.1 (2018).

BibTeX: 

Last updated on 2019-28-02 at 06:24