Plasma gelsolin promotes re-epithelialization

Wittmann J, Dieckow J, Schröder H, Hampel U, Garreis F, Jacobi C, Milczarek A, Hsieh KL, Pulli B, Chen JW, Hoogeboom S, Bräuer L, Paulsen F, Schob S, Schicht M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 8

Journal Issue: 1

DOI: 10.1038/s41598-018-31441-2

Abstract

Woundhealing disorders characterized by impaired or delayed re-epithelialization are a serious medical problem that is painful and difficult to treat. Gelsolin (GSN), a known actin modulator, supports epithelial cell regeneration and apoptosis. The aim of this study was to estimate the potential of recombinant gelsolin (rhu-pGSN) for ocular surface regeneration to establish a novel therapy for delayed or complicated wound healing. We analyzed the influence of gelsolin on cell proliferation and wound healing in vitro, in vivo/ex vivo and by gene knockdown. Gelsolin is expressed in all tested tissues of the ocular system as shown by molecular analysis. The concentration of GSN is significantly increased in tear fluid samples of patients with dry eye disease. rhu-pGSN induces cell proliferation and faster wound healing in vitro as well as in vivo/ex vivo. TGF-β dependent transcription of SMA is significantly decreased after GSN gene knockdown. Gelsolin is an inherent protein of the ocular system and is secreted into the tear fluid. Our results show a positive effect on corneal cell proliferation and wound healing. Furthermore, GSN regulates the synthesis of SMA in myofibroblasts, which establishes GSN as a key protein of TGF-β dependent cell differentiation.

Authors with CRIS profile

Johannes Wittmann Lehrstuhl für Funktionelle und Klinische Anatomie Henrik Schröder Professur für Oberflächenaktive Proteine Ulrike Hampel Lehrstuhl für Funktionelle und Klinische Anatomie Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Christina Jacobi Medizinische Fakultät Aleksandra Milczarek Lehrstuhl für Funktionelle und Klinische Anatomie Lars Bräuer Professur für Oberflächenaktive Proteine Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

Universität Leipzig DE Germany (DE) Taipei Medical University Hospital (TMUH) / 臺北醫學大學附設醫院 TW Taiwan (TW) Massachusetts General Hospital US United States (USA) (US) Fraunhofer-Institut für Integrierte Schaltungen (IIS) DE Germany (DE)

How to cite

APA:

Wittmann, J., Dieckow, J., Schröder, H., Hampel, U., Garreis, F., Jacobi, C.,... Schicht, M. (2018). Plasma gelsolin promotes re-epithelialization. Scientific Reports, 8(1). https://dx.doi.org/10.1038/s41598-018-31441-2

MLA:

Wittmann, Johannes, et al. "Plasma gelsolin promotes re-epithelialization." Scientific Reports 8.1 (2018).

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