Expression and Regulation of S100 Fused-Type Protein Hornerin at the Ocular Surface and Lacrimal Apparatus

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Details zur Publikation

Autorinnen und Autoren: Garreis F, Jahn J, Wild K, Abrar D, Schicht M, Schroeder JM, Paulsen F
Zeitschrift: Investigative Ophthalmology & Visual Science
Jahr der Veröffentlichung: 2017
Band: 58
Heftnummer: 13
Seitenbereich: 5968-5977
ISSN: 0146-0404
eISSN: 1552-5783


Abstract

Purpose: The S100 fused-type proteins hornerin (HRNR) and filaggrin-2 (FLG2) are members of the epidermal differentiation complex, which is involved in terminal differentiation of keratinocytes via cornification as well as maintenance of the epidermal antimicrobial barrier. We investigated the expression and possible regulation of HRNR and FLG2 at the ocular surface and in the lacrimal apparatus.
Methods: Tissues of the lacrimal apparatus and ocular surface were analyzed systematically by means of RT-PCR, immunohistochemistry, and immuntransmission electron microscopy (iTEM) for their ability to express and produce HRNR and FLG2. In addition, inducibility and regulation of HRNR were studied in cultivated human corneal (HCE), conjunctival (HCjE), as well as meibomian gland (HMGEC) epithelial cell line by real-time RT-PCR.
Results: RT-PCR, immunohistochemistry, and iTEM revealed constitutive expression of HRNR in the epithelium of cornea, conjunctiva, nasolacrimal ducts, and acinus cells of lacrimal and meibomian glands. HRNR also was detected in tears of healthy volunteers. No expression of FLG2 could be detected in tissue samples of the ocular surface and lacrimal apparatus. Real-time RT-PCR revealed a decreased HRNR gene expression after challenge with proinflammatory cytokines and supernatants of Escherichia coli and Pseudomonas aeroginosa in HCE cells, whereas HCjE cells revealed no changes. In HMGECs serum-induced differentiation and application of all-trans retinoic acid significantly increased HRNR gene expression.
Conclusions: The data suggest that HRNR, but not FLG2, is a component of the ocular surface and lacrimal apparatus, including meibomian glands. HRNR seems to contribute to the maintenance of the epithelial barrier at the ocular surface and, thus, also may be involved in ocular surface diseases.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Abrar, Daniel
Lehrstuhl für Funktionelle und Klinische Anatomie
Garreis, Fabian PD Dr.
Lehrstuhl für Funktionelle und Klinische Anatomie
Paulsen, Friedrich Prof. Dr.
Lehrstuhl für Funktionelle und Klinische Anatomie
Schicht, Martin Dr.
Lehrstuhl für Funktionelle und Klinische Anatomie
Wild, Katharina
Lehrstuhl für Funktionelle und Klinische Anatomie


Einrichtungen weiterer Autorinnen und Autoren

Universitätsklinikum Schleswig-Holstein (UKSH)


Zitierweisen

APA:
Garreis, F., Jahn, J., Wild, K., Abrar, D., Schicht, M., Schroeder, J.-M., & Paulsen, F. (2017). Expression and Regulation of S100 Fused-Type Protein Hornerin at the Ocular Surface and Lacrimal Apparatus. Investigative Ophthalmology & Visual Science, 58(13), 5968-5977. https://dx.doi.org/10.1167/iovs.17-22637

MLA:
Garreis, Fabian, et al. "Expression and Regulation of S100 Fused-Type Protein Hornerin at the Ocular Surface and Lacrimal Apparatus." Investigative Ophthalmology & Visual Science 58.13 (2017): 5968-5977.

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Zuletzt aktualisiert 2019-20-09 um 15:48