IL-1 beta as mucosal vaccine adjuvant: the specific induction of tissue-resident memory T cells improves the heterosubtypic immunity against influenza A viruses

Lapuente D, Bonsmann MSG, Maaske A, Stab V, Heinecke V, Watzstedt K, Hess R, Westendorf AM, Bayer W, Ehrhardt C, Tenbusch M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Mucosal Immunology Nature Publishing Group: Open Access Hybrid Model Option A

Book Volume: 11

Pages Range: 1265-1278

Journal Issue: 4

DOI: 10.1038/s41385-018-0017-4

Abstract

A universal influenza vaccine must provide protection against antigenically divergent influenza viruses either through broadly neutralizing antibodies or cross-reactive T cells. Here, intranasal immunizations with recombinant adenoviral vectors (rAd) encoding hemagglutinin (HA) and nucleoprotein (NP) in combination with rAd-Interleukin-(IL)-1 beta or rAd-IL-18 were evaluated for their efficacy in BALB/c mice. Mucosal delivery of rAd-IL-1 beta enhanced HA-specific antibody responses including strain-specific neutralizing antibodies. Nevertheless, the beneficial effects on the local T cell responses were much more impressive reflected by increased numbers of CD103(+)CD69(+) tissue-resident memory T cells (T-RM). This increased immunogenicity translated into superior protection against infections with homologous and heterologous strains including H1N1, pH1N1, H3N2, and H7N7. Inhibition of the egress of circulating T cells out of the lymph nodes during the heterologous infection had no impact on the degree of protection underscoring the unique potential of T-RM for the local containment of mucosal infections. The local co-expression of IL-1 beta and antigen lead to the activation of critical checkpoints in the formation of T-RM including activation of epithelial cells, expression of chemokines and adhesion molecules, recruitment of lung-derived CD103(+) DCs, and finally local T-RM imprinting. Given the importance of T-RM-mediated protection at mucosal barriers, this study has major implications for vaccine development.

Authors with CRIS profile

Matthias Tenbusch Professur für Genbasierte Immunisierungsverfahren

Involved external institutions

Ruhr-Universität Bochum (RUB) DE Germany (DE) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE) Universität Duisburg-Essen (UDE) DE Germany (DE)

How to cite

APA:

Lapuente, D., Bonsmann, M.S.G., Maaske, A., Stab, V., Heinecke, V., Watzstedt, K.,... Tenbusch, M. (2018). IL-1 beta as mucosal vaccine adjuvant: the specific induction of tissue-resident memory T cells improves the heterosubtypic immunity against influenza A viruses. Mucosal Immunology, 11(4), 1265-1278. https://doi.org/10.1038/s41385-018-0017-4

MLA:

Lapuente, D., et al. "IL-1 beta as mucosal vaccine adjuvant: the specific induction of tissue-resident memory T cells improves the heterosubtypic immunity against influenza A viruses." Mucosal Immunology 11.4 (2018): 1265-1278.

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