Generation of T follicular helper cells in vitro: requirement for B-cell receptor cross-linking and cognate B- and T-cell interaction

Kolenbrander A, Grewe B, Nemazee D, Überla K, Temchura V (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Immunology Wiley-Blackwell

Book Volume: 153

Pages Range: 214-224

Journal Issue: 2

DOI: 10.1111/imm.12834

Abstract

The minimum requirements for invitro modelling of natural CD4(+) T-cell differentiation into T follicular helper (Tfh) cells are still under investigation. We co-cultured wild-type and T-cell receptor (TCR) transgenic CD4(+) T cells from naive mice with dendritic cells and B-cell receptor (BCR) transgenic B cells in the presence of HIV-derived virus-like particles containing matched B-cell and T-cell epitopes. This co-culturing induced co-expression of Tfh-master regulator transcription factor BCL-6 and CXCR5 in up to 10% of the wild-type and up to 40% of the TCR-transgenic CD4(+) T cells. Phenotypic markers, production of interleukin-21 and isotype switching of the B cells to IgG1 further indicated a helper function of the induced Tfh cells invitro. Dendritic cells supported the generation of functional Tfh cells, but were unable to induce them without cognate B cells. Hence, our study presents a robust experimental system for efficient generation of functionally active Tfh cells invitro and confirms the importance of cognate B- and T-cell cross-talk for the Tfh differentiation process.

Authors with CRIS profile

Klaus Überla Institute of Clinical and Molecular Virology Vladimir Temchura Institute of Clinical and Molecular Virology

Involved external institutions

Ruhr-Universität Bochum (RUB) DE Germany (DE) Scripps Research Institute (TSRI) US United States (USA) (US)

How to cite

APA:

Kolenbrander, A., Grewe, B., Nemazee, D., Überla, K., & Temchura, V. (2018). Generation of T follicular helper cells in vitro: requirement for B-cell receptor cross-linking and cognate B- and T-cell interaction. Immunology, 153(2), 214-224. https://doi.org/10.1111/imm.12834

MLA:

Kolenbrander, Anne, et al. "Generation of T follicular helper cells in vitro: requirement for B-cell receptor cross-linking and cognate B- and T-cell interaction." Immunology 153.2 (2018): 214-224.

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