Functionalization of T lymphocytes for magnetically controlled immune therapy: Selection of suitable superparamagnetic iron oxide nanoparticles

Conference contribution


Publication Details

Author(s): Mühlberger M, Janko C, Unterweger H, Schreiber E, Band J, Lehmann C, Dudziak D, Lee G, Alexiou C, Tietze R
Publication year: 2019
Volume: 473
Pages range: 61-67
ISSN: 0304-8853


Abstract

According to the World Health Organization, cancer is the second most important cause of death in Europe. Due to its manifold manifestations, it is not possible to treat all patients according to a uniform scheme. However, all solid tumors have one thing in common: independent of the tumor's molecular subgroup and the treatment protocol, the immune status of the tumor, especially the amount of tumor infiltrating lymphocytes (TILs), is important for the patient's clinical outcome - the higher the number of TILs, the better the outcome. For this reason it seems desirable to increase the number of TILs.
One way to accumulate T cells in the tumor area is to make them magnetizable and attract them with an external magnetic field. Magnetization can be achieved by superparamagnetic iron oxide nanoparticles (SPIONs) which can be bound to the cells' surface or internalized into the cells.
For this study, SPIONs with different coatings were synthesized and incubated with immortalized mouse T lymphocytes. SPIONs only stabilized with lauric acid (LA) coated in situ or afterwards showed high toxicity. Addition of an albumin layer increased the biocompatibility but reduced cellular uptake. To increase the cellular uptake the albumin coated particles were aminated, leading to both higher uptake and toxicity, dependent on the degree of amination. In the presence of an externally applied magnetic field, T cells loaded with selected types and amounts of SPIONs were guidable.
With this promising pilot study we already can demonstrate that it is possible to attract SPION bearing T cells by an external magnet.
To sum up, biocompatibility and uptake of SPIONs by T cells are opposing events. Thus, for the functionalization of T cells with SPIONs the balance between uptake and toxicity must be evaluated carefully.


FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Dudziak, Diana Prof. Dr.
Professur für die Biologie Dendritischer Zellen
Janko, Christina Dr. rer. nat.
Einrichtungen, die zum Universitätsklinikum Erlangen gehören
Lee, Geoffrey Prof.
Lehrstuhl für Pharmazeutische Technologie
Lehmann, Christian Dr. rer. nat.
Hautklinik
Mühlberger, Marina
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Tietze, Rainer Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


How to cite

APA:
Mühlberger, M., Janko, C., Unterweger, H., Schreiber, E., Band, J., Lehmann, C.,... Tietze, R. (2019). Functionalization of T lymphocytes for magnetically controlled immune therapy: Selection of suitable superparamagnetic iron oxide nanoparticles. (pp. 61-67).

MLA:
Mühlberger, Marina, et al. "Functionalization of T lymphocytes for magnetically controlled immune therapy: Selection of suitable superparamagnetic iron oxide nanoparticles." 2019. 61-67.

BibTeX: 

Last updated on 2019-08-02 at 12:38