Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

Journal article


Publication Details

Author(s): Bilyy R, Unterweger H, Weigel B, Dumych T, Paryzhak S, Vovk V, Liao Z, Alexiou C, Herrmann M, Janko C
Journal: Frontiers in Immunology
Publication year: 2018
Volume: 9
ISSN: 1664-3224


Abstract

If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis.


FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Herrmann, Martin Prof. Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


External institutions
Danylo Halytsky Lviv National Medical University / Львiвський Національний Медичний Унiверситет iм. Данила Галицького


How to cite

APA:
Bilyy, R., Unterweger, H., Weigel, B., Dumych, T., Paryzhak, S., Vovk, V.,... Janko, C. (2018). Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps. Frontiers in Immunology, 9. https://dx.doi.org/10.3389/fimmu.2018.02266

MLA:
Bilyy, Rostyslav, et al. "Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps." Frontiers in Immunology 9 (2018).

BibTeX: 

Last updated on 2019-17-04 at 08:18