Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat

Journal article


Publication Details

Author(s): Prochnicki A, Amann KU, Wegner M, Sock E, Pfister E, Shankland S, Pippin J, Daniel C
Journal: American Journal of Pathology
Publication year: 2018
Volume: 188
Journal issue: 11
Pages range: 2529-2541
ISSN: 0002-9440


Abstract

Mechanisms of glomerular crescent formation and podocyte repair processes are still unclear. Therefore, we investigated the expression of the transcription factor Sox9 as a potential marker of a subpopulation of parietal epithelial cells (PECs) with potential regenerative properties. Glomerular Sox9 expression was characterized in detail in a rat anti-glomerular basement membrane (GBM) nephritis model using immunofluorescence and confocal laser scanning microscopy. In healthy kidneys Sox9 is expressed in a subpopulation of PECs restricted to approximately 20% to 50% of PEC nuclei and was highly conserved in all investigated species. During rat anti-GBM nephritis the number of glomerular Sox9+ cells increased and was associated with proliferation activity. In nephritic glomeruli Sox9 expression was not restricted to Bowman's capsule lining but was also found on cells of the glomerular tuft. Nearly all Sox9+ cells also expressed the PEC marker Pax8, whereas endothelial cells, mesangial cells, macrophages, and T lymphocytes lacked Sox9 expression. At the margins of crescents Sox9+/Pax8+ cells additionally expressed podocyte markers. In contrast, in sclerotic lesions a minority of Sox9+/Pax8+ cells expressed the myofibroblast marker α-smooth muscle actin. In glomerular Sox9+ cells Jagged 1 was up-regulated. During anti-GBM nephritis Sox9+ PECs proliferate and migrate onto the glomerular tuft. Future studies are needed to confirm the origin of Sox9+ cells from PECs and differentiation in both podocytes and/or myofibroblasts.


FAU Authors / FAU Editors

Amann, Kerstin Ute Prof. Dr.
Nephropathologische Abteilung im Pathologischen Institut
Daniel, Christoph Prof. Dr.
Nephropathologische Abteilung im Pathologischen Institut
Sock, Elisabeth Prof. Dr.
Lehrstuhl für Biochemie und Pathobiochemie
Wegner, Michael Prof. Dr.
Lehrstuhl für Biochemie und Pathobiochemie


External institutions with authors

University of Washington


How to cite

APA:
Prochnicki, A., Amann, K.U., Wegner, M., Sock, E., Pfister, E., Shankland, S.,... Daniel, C. (2018). Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat. American Journal of Pathology, 188(11), 2529-2541. https://dx.doi.org/10.1016/j.ajpath.2018.07.023

MLA:
Prochnicki, Ania, et al. "Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat." American Journal of Pathology 188.11 (2018): 2529-2541.

BibTeX: 

Last updated on 2019-04-02 at 08:23