Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Keller KE, Bhattacharya SK, Borras T, Brunner TM, Chansangpetch S, Clark AF, Dismuke WM, Du Y, Elliott MH, Ethier CR, Faralli JA, Freddo TF, Fuchshofer R, Giovingo M, Gong H, Gonzalez P, Huang A, Johnstone MA, Kaufman PL, Kelley MJ, Knepper PA, Kopczynski CC, Kuchtey JG, Kuchtey RW, Kuehn MH, Lieberman RL, Lin SC, Liton P, Liu Y, Lütjen-Drecoll E, Mao W, Masis-Solano M, Mcdonnell F, Mcdowell CM, Overby DR, Pattabiraman PP, Raghunathan VK, Rao PV, Rhee DJ, Chowdhury UR, Russell P, Samples JR, Schwartz D, Stubbs EB, Tamm ER, Tan JC, Toris CB, Torrejon KY, Vranka JA, Wirtz MK, Yorio T, Zhang J, Zode GS, Fautsch MP, Peters DM, Acott TS, Stamer WD (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Book Volume: 171

Pages Range: 164-173

DOI: 10.1016/j.exer.2018.03.001

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Authors with CRIS profile

Involved external institutions

University of Miami United States (USA) (US) Oregon Health and Science University (OSHU) United States (USA) (US) University of Illinois at Urbana-Champaign United States (USA) (US) Aerie Pharmaceuticals Inc. United States (USA) (US) University of Oklahoma United States (USA) (US) John H. Stroger Jr. Hospital of Cook County United States (USA) (US) University of Wisconsin - Madison United States (USA) (US) University of Washington United States (USA) (US) Boston University United States (USA) (US) Glaucoma Research Foundation United States (USA) (US) Universität Regensburg Germany (DE) Mayo Clinic United States (USA) (US) University of California Davis (UCDAVIS) United States (USA) (US) University of North Texas (UNT) United States (USA) (US) University of California San Francisco (UCSF) United States (USA) (US) EyeSonix United States (USA) (US) Duke University United States (USA) (US) Loyola University Chicago United States (USA) (US) Case Western Reserve University United States (USA) (US) Vanderbilt University United States (USA) (US) University of California Los Angeles (UCLA) United States (USA) (US) University of Pittsburgh United States (USA) (US) Massachusetts College of Pharmacy and Health Sciences (MCPHS University) United States (USA) (US) Imperial College London / The Imperial College of Science, Technology and Medicine United Kingdom (GB) University of North Carolina at Chapel Hill United States (USA) (US) Georgia Institute of Technology United States (USA) (US) University of Southern California (USC) United States (USA) (US) University of Iowa United States (USA) (US) Augusta University United States (USA) (US) Glauconix Biosciences, Inc. United States (USA) (US) University of Houston United States (USA) (US)

How to cite

APA:

Keller, K.E., Bhattacharya, S.K., Borras, T., Brunner, T.M., Chansangpetch, S., Clark, A.F.,... Stamer, W.D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental Eye Research, 171, 164-173. https://dx.doi.org/10.1016/j.exer.2018.03.001

MLA:

Keller, Kate E., et al. "Consensus recommendations for trabecular meshwork cell isolation, characterization and culture." Experimental Eye Research 171 (2018): 164-173.

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