Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Keller KE, Bhattacharya SK, Borras T, Brunner TM, Chansangpetch S, Clark AF, Dismuke WM, Du Y, Elliott MH, Ethier CR, Faralli JA, Freddo TF, Fuchshofer R, Giovingo M, Gong H, Gonzalez P, Huang A, Johnstone MA, Kaufman PL, Kelley MJ, Knepper PA, Kopczynski CC, Kuchtey JG, Kuchtey RW, Kuehn MH, Lieberman RL, Lin SC, Liton P, Liu Y, Lütjen-Drecoll E, Mao W, Masis-Solano M, Mcdonnell F, Mcdowell CM, Overby DR, Pattabiraman PP, Raghunathan VK, Rao PV, Rhee DJ, Chowdhury UR, Russell P, Samples JR, Schwartz D, Stubbs EB, Tamm ER, Tan JC, Toris CB, Torrejon KY, Vranka JA, Wirtz MK, Yorio T, Zhang J, Zode GS, Fautsch MP, Peters DM, Acott TS, Stamer WD
Zeitschrift: Experimental Eye Research
Jahr der Veröffentlichung: 2018
Band: 171
Seitenbereich: 164-173
ISSN: 0014-4835


Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.


FAU-Autoren / FAU-Herausgeber

Lütjen-Drecoll, Elke Prof. Dr.
Lehrstuhl für funktionelle und klinische Anatomie


Autor(en) der externen Einrichtung(en)
Aerie Pharmaceuticals Inc.
Augusta University
Boston University
Case Western Reserve University
Duke University
EyeSonix
Georgia Institute of Technology
Glaucoma Research Foundation
Glauconix Biosciences, Inc.
Imperial College London / The Imperial College of Science, Technology and Medicine
John H. Stroger Jr. Hospital of Cook County
Loyola University Chicago
Massachusetts College of Pharmacy and Health Sciences (MCPHS University)
Mayo Clinic
Oregon Health and Science University (OSHU)
Universität Regensburg
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University of California San Francisco (UCSF)
University of Houston
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University of Iowa
University of Miami
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University of North Texas (UNT)
University of Oklahoma
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University of Southern California (USC)
University of Washington
University of Wisconsin - Madison
Vanderbilt University


Zitierweisen

APA:
Keller, K.E., Bhattacharya, S.K., Borras, T., Brunner, T.M., Chansangpetch, S., Clark, A.F.,... Stamer, W.D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental Eye Research, 171, 164-173. https://dx.doi.org/10.1016/j.exer.2018.03.001

MLA:
Keller, Kate E., et al. "Consensus recommendations for trabecular meshwork cell isolation, characterization and culture." Experimental Eye Research 171 (2018): 164-173.

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Zuletzt aktualisiert 2019-04-02 um 13:53