Biofabrication of vessel-like structures with alginate di-aldehyde-gelatin (ADA-GEL) bioink.

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Ruther F, Distler T, Boccaccini AR, Detsch R
Zeitschrift: Journal of Materials Science-Materials in Medicine
Jahr der Veröffentlichung: 2018
Band: 30
Heftnummer: 1
Seitenbereich: 8
ISSN: 0957-4530


One of the key challenges in the field of blood vessel engineering is the in vitro production of small and large diameter vessels. Considering that a combination of alginate di-aldehyde and gelatin (ADA-GEL) has been successfully applied for different biofabrication approaches, the aim of this study was to exploit ADA-GEL for the fabrication of vessel structures with diameters up to 4 mm. To explore plotting possibilities and to study the swelling behaviour, a library of vessel-like constructs with different diameters made from 2, 3 and 4% (w/v) alginate was created by using various hand-crafted double-needle extrusion systems. Vessel diameters were varied through changes of the double-needle core and outer diameters. A straightforward model for the production of vessel of different diameters from a variety of double-needle systems was established and vessel-constructs with diameters of up to 3.7 mm could be created. It was successfully demonstrated that an artificial vessel, consisting of an outer layer of 7.5% ADA50-GEL50 and an inner core of 3% gelatin, can support the proliferation and migration of an immobilized co-culture containing fibroblast (NHDF) and endothelial (HUVEC) cells. The openness and tightness of the hollow ADA-GEL structures were further confirmed by a dye injection test. Nanoindentation was performed to determine the Young's modulus of the used materials. Cell vitality was proved after 1, 2 and 3 weeks of incubation. The results showed a nearly twofold increase of viable cells per week. Fluorescent images confirmed cell migration during the whole incubation time.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Boccaccini, Aldo R. Prof. Dr.-Ing.
Ruther, Florian
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Detsch, Rainer Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Distler, Thomas
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)


Ruther, F., Distler, T., Boccaccini, A.R., & Detsch, R. (2018). Biofabrication of vessel-like structures with alginate di-aldehyde-gelatin (ADA-GEL) bioink. Journal of Materials Science-Materials in Medicine, 30(1), 8.

Ruther, Florian, et al. "Biofabrication of vessel-like structures with alginate di-aldehyde-gelatin (ADA-GEL) bioink." Journal of Materials Science-Materials in Medicine 30.1 (2018): 8.


Zuletzt aktualisiert 2019-28-01 um 23:08