3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane.

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Borowiec J, Hampl J, Singh S, Haefner S, Friedel K, Mai P, Brauer D, Ruther F, Liverani L, Boccaccini AR, Schober A
Zeitschrift: ACS Applied Materials and Interfaces
Jahr der Veröffentlichung: 2018
Band: 10
Heftnummer: 26
Seitenbereich: 22857-22865
ISSN: 1944-8244


Abstract

Micrometer-scale biochemical or topographical patterning is commonly used to guide the cell attachment and growth, but the ability to combine these patterns into an integrated surface with defined chemical and geometrical characteristics still remains a technical challenge. Here, we present a technical solution for simultaneous construction of 3D morphologies, in the form of channels, on porous membranes along with precise transfer of extracellular matrix proteins into the channels to create patterns with geometrically restricting features. By combining the advantages of microthermoforming and microcontact printing, this technique offers a unique patterning process that provides spatiotemporal control over morphological and chemical feature in a single step. By use of our 3D-microcontact printing (3DμCP), determined microstructures like channels with different depths and widths even with more complex patterns can be fabricated. Collagen, fibronectin, and laminin were successfully transferred inside the predesigned geometries, and the validity of the process was confirmed by antibody staining. Cells cultivated on 3DμCP patterned polycarbonate membrane have shown selective adhesion and growth. This technique offers a novel tool for creating freeform combinatorial patterning on the thermoformable surface.


FAU-Autoren / FAU-Herausgeber

Boccaccini, Aldo R. Prof. Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Liverani, Liliana Dr.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Ruther, Florian
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)


Autor(en) der externen Einrichtung(en)
Technische Universität Ilmenau


Zitierweisen

APA:
Borowiec, J., Hampl, J., Singh, S., Haefner, S., Friedel, K., Mai, P.,... Schober, A. (2018). 3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane. ACS Applied Materials and Interfaces, 10(26), 22857-22865. https://dx.doi.org/10.1021/acsami.8b06585

MLA:
Borowiec, Justyna, et al. "3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane." ACS Applied Materials and Interfaces 10.26 (2018): 22857-22865.

BibTeX: 

Zuletzt aktualisiert 2019-20-03 um 11:38