Label-Free High-Throughput Leukemia Detection by Holographic Microscopy

Journal article

Publication Details

Author(s): Ugele M, Weniger M, Stanzel M, Bassler M, Krause S, Friedrich O, Hayden O, Richter L
Journal: Advanced Science
Publication year: 2018
Volume: 5
Journal issue: 12
ISSN: 2198-3844


Complete blood count and differentiation of leukocytes (DIFF) belong to the most frequently performed laboratory diagnostic tests. Here, a flow cytometry-based method for label-free DIFF of untouched leukocytes by digital holographic microscopy on the rich phase contrast of peripheral leukocyte images, using highly controlled 2D hydrodynamic focusing conditions is reported. Principal component analysis of morphological characteristics of the reconstructed images allows classification of nine leukocyte types, in addition to different types of leukemia and demonstrates disappearance of acute myeloid leukemia cells in remission. To exclude confounding effects, the classification strategy is tested by the analysis of 20 blinded clinical samples. Here, 70% of the specimens are correctly classified with further 20% classifications close to a correct diagnosis. Taken together, the findings indicate a broad clinical applicability of the cytometry method for automated and reagent-free diagnosis of hematological disorders.

FAU Authors / FAU Editors

Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie
Krause, Stefan Prof. Dr.
Medizinische Klinik 5 - Hämatologie und Internistische Onkologie

External institutions with authors

Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Siemens AG, Healthcare Sector

How to cite

Ugele, M., Weniger, M., Stanzel, M., Bassler, M., Krause, S., Friedrich, O.,... Richter, L. (2018). Label-Free High-Throughput Leukemia Detection by Holographic Microscopy. Advanced Science, 5(12).

Ugele, Matthias, et al. "Label-Free High-Throughput Leukemia Detection by Holographic Microscopy." Advanced Science 5.12 (2018).


Last updated on 2019-24-01 at 02:08