Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Dulin D, Bauer DLV, Malinen AM, Bakermans JJW, Kaller M, Morichaud Z, Petushkov I, Depken M, Brodolin K, Brodolin K, Kulbachinskiy A, Kapanidis AN
Zeitschrift: Nature Communications
Jahr der Veröffentlichung: 2018
Band: 9
Heftnummer: 1
ISSN: 2041-1723


Abstract

Transcription in bacteria is controlled by multiple molecular mechanisms that precisely regulate gene expression. It has been recently shown that initial RNA synthesis by the bacterial RNA polymerase (RNAP) is interrupted by pauses; however, the pausing determinants and the relationship of pausing with productive and abortive RNA synthesis remain poorly understood. Using single-molecule FRET and biochemical analysis, here we show that the pause encountered by RNAP after the synthesis of a 6-nt RNA (ITC6) renders the promoter escape strongly dependent on the NTP concentration. Mechanistically, the paused ITC6 acts as a checkpoint that directs RNAP to one of three competing pathways: productive transcription, abortive RNA release, or a new unscrunching/scrunching pathway. The cyclic unscrunching/scrunching of the promoter generates a long-lived, RNA-bound paused state; the abortive RNA release and DNA unscrunching are thus not as tightly linked as previously thought. Finally, our new model couples the pausing with the abortive and productive outcomes of initial transcription.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Dulin, David Dr.
Interdisziplinäres Zentrum für Klinische Forschung IZKF Nachwuchsgruppe II Dr.Sorokin


Einrichtungen weiterer Autorinnen und Autoren

Delft University of Technology
Russian Academy of Sciences / Росси́йская акаде́мия нау́к (RAS)
University of Montpellier / Université Montpellier
University of Oxford


Zitierweisen

APA:
Dulin, D., Bauer, D.L.V., Malinen, A.M., Bakermans, J.J.W., Kaller, M., Morichaud, Z.,... Kapanidis, A.N. (2018). Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria. Nature Communications, 9(1). https://dx.doi.org/10.1038/s41467-018-03902-9

MLA:
Dulin, David, et al. "Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria." Nature Communications 9.1 (2018).

BibTeX: 

Zuletzt aktualisiert 2019-23-01 um 08:38