B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

Fecher P, Caspell R, Naeem V, Karulin AY, Kürten S, Lehmann P (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Cells MDPI

Book Volume: 7

Journal Issue: 6

DOI: 10.3390/cells7060050

Abstract

In individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the serum. Long term humoral immunity is, however, also critically dependent on the ability of memory B cells to engage in a secondary antibody response upon re-exposure to the antigen. Antibody molecules in the body are short lived, having a half-life of weeks, while memory B cells have a life span of decades. Therefore, the presence of serum antibodies is not always a reliable indicator of B cell memory and comprehensive monitoring of humoral immunity requires that both serum antibodies and memory B cells be assessed. The prevailing view is that resting memory B cells and B cell blasts in peripheral blood mononuclear cells (PBMC) cannot be cryopreserved without losing their antibody secreting function, and regulated high throughput immune monitoring of B cell immunity is therefore confined to-and largely limited by-the need to test freshly isolated PBMC. Using optimized protocols for freezing and thawing of PBMC, and four color ImmunoSpot® analysis for the simultaneous detection of all immunoglobulin classes/subclasses we show here that both resting memory B cells and B cell blasts retain their ability to secrete antibody after thawing, and thus demonstrate the feasibility of B cell immune monitoring using cryopreserved PBMC.

Authors with CRIS profile

Stefanie Kürten Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie

Involved external institutions

CTL Europe GmbH DE Germany (DE)

How to cite

APA:

Fecher, P., Caspell, R., Naeem, V., Karulin, A.Y., Kürten, S., & Lehmann, P. (2018). B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody. Cells, 7(6). https://dx.doi.org/10.3390/cells7060050

MLA:

Fecher, Philipp, et al. "B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody." Cells 7.6 (2018).

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