Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering

Goldberg-Bockhorn E, Schwarz S, Subedi R, Elsässer A, Riepl R, Walther P, Körber L, Breiter R, Stock K, Rotter N (2018)

Publication Status: Published

Publication Type: Journal article

Publication year: 2018

Journal

Lasers in Medical Science Springer Verlag (Germany)

Publisher: SPRINGER LONDON LTD

Book Volume: 33

Pages Range: 375-384

Journal Issue: 2

DOI: 10.1007/s10103-017-2402-8

Abstract

The implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by carbon dioxide (CO2) and Er:YAG laser application to facilitate the migration of chondrocytes inside the scaffold. After laser treatment, the scaffolds were seeded with human nasal septal chondrocytes and analyzed with respect to cell migration and formation of new extracellular matrix proteins. Histology, immunohistochemistry, SEM, and TEM examination revealed an increase of the scaffolds' surface area with proliferation of cell numbers on the scaffolds for both laser types. The lack of cytotoxic effects was demonstrated by standard cytotoxicity testing. However, a thermal denaturation area seemed to hinder the migration of the chondrocytes inside the scaffolds, even more so after CO2 laser treatment. Therefore, the Er:YAG laser seemed to be better suitable. Further modifications of the laser adjustments or the use of alternative laser systems might be advantageous for surface enlargement and to facilitate migration of chondrocytes into the scaffold in one step.

Authors with CRIS profile

Ludwig Körber Lehrstuhl für Bioverfahrenstechnik Roman Breiter Lehrstuhl für Bioverfahrenstechnik

Involved external institutions

Universität Ulm DE Germany (DE) Universitätsklinikum Ulm DE Germany (DE) Universitätsklinikum Mannheim DE Germany (DE) Paracelsus Medizinische Privatuniversität, Nürnberg DE Germany (DE)

How to cite

APA:

Goldberg-Bockhorn, E., Schwarz, S., Subedi, R., Elsässer, A., Riepl, R., Walther, P.,... Rotter, N. (2018). Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering. Lasers in Medical Science, 33(2), 375-384. https://dx.doi.org/10.1007/s10103-017-2402-8

MLA:

Goldberg-Bockhorn, Eva, et al. "Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering." Lasers in Medical Science 33.2 (2018): 375-384.

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