ISCEV extended protocol for the photopic negative response (PhNR) of the full-field electroretinogram

Journal article

Publication Details

Author(s): Frishman L, Sustar M, Kremers J, Mcanany JJ, Sarossy M, Tzekov R, Viswanathan S
Journal: Documenta Ophthalmologica
Publication year: 2018
Volume: 136
Journal issue: 3
Pages range: 207-211
ISSN: 0012-4486


The International Society for Clinical Electrophysiology of Vision (ISCEV) Standard for full-field electroretinography (ERG) describes a minimum procedure, but encourages more extensive testing. This ISCEV extended protocol describes an extension to the ERG Standard, namely the photopic negative response (PhNR) of the light-adapted flash ERG, as a well-established technique that is broadly accepted by experts in the field. The PhNR is a slow negative-going wave after the b-wave that provides information about the function of retinal ganglion cells and their axons. The PhNR can be reduced in disorders that affect the innermost retina, including glaucoma and other forms of optic neuropathy. This document, based on existing literature, provides a protocol for recording and analyzing the PhNR in response to a brief flash. The protocol includes full-field stimulation, a frequency bandwidth of the recording in which the lower limit does not exceed 0.3 Hz, and a spectrally narrowband stimulus, specifically, a red flash on a rod saturating blue background. Suggested flash strengths cover a range up to and including the minimum required to elicit a maximum amplitude PhNR. This extended protocol for recording the PhNR provides a simple test of generalized retinal ganglion cell function that could be added to standard ERG testing.

FAU Authors / FAU Editors

Kremers, Jan Prof. Dr.
Professur für Experimentelle Augenheilkunde

External institutions with authors

Ljubljana University Medical Centre (Ljubljana UMC) / Univerzitetni klinični center Ljubljana
SUNY Downstate Medical Center
The University of Melbourne
University of Houston
University of Illinois at Urbana-Champaign
University of South Florida (USF)

How to cite

Frishman, L., Sustar, M., Kremers, J., Mcanany, J.J., Sarossy, M., Tzekov, R., & Viswanathan, S. (2018). ISCEV extended protocol for the photopic negative response (PhNR) of the full-field electroretinogram. Documenta Ophthalmologica, 136(3), 207-211.

Frishman, Laura, et al. "ISCEV extended protocol for the photopic negative response (PhNR) of the full-field electroretinogram." Documenta Ophthalmologica 136.3 (2018): 207-211.


Last updated on 2019-07-01 at 00:10