Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS)

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Gebrekidan M, Erber R, Hartmann A, Fasching P, Emons J, Beckmann M, Bräuer A
Zeitschrift: Technology in Cancer Research & Treatment
Jahr der Veröffentlichung: 2018
Band: 17
ISSN: 1533-0346


Abstract

We used a shifted-excitation Raman difference spectroscopy method for the ex vivo classification of resected and formalin-fixed breast tissue samples as normal (healthy) tissue, fibroadenoma, or invasive carcinoma. We analyzed 8 tissue samples containing invasive carcinoma that were surrounded by normal tissue and 3 tissue samples with fibroadenoma only. We made various measurement sites on various tissue samples, in total 240 measurements for each type of tissue. Although the acquired raw spectra contain enough information to clearly differentiate between normal and tumor (fibroadenoma and invasive carcinoma) tissue, the differentiation between fibroadenoma and invasive carcinoma was possible only after the shifted-excitation Raman difference spectroscopy isolation of pure Raman spectra from the heavily fluorescence interfered raw spectra. We used 784 and 785 nm as excitation wavelengths for the shifted-excitation Raman difference spectroscopy method. The differences in the obtained pure Raman spectra are assigned to the different chemical compositions of normal breast tissue, fibroadenoma, and invasive breast carcinoma. Principal component analysis and linear discriminant analysis showed excellent classification results in the Raman shift range between 1000 and 1800 cm-1. Invasive breast carcinoma was identified with 99.15% sensitivity, and the absence of invasive carcinoma was identified with 90.40% specificity. Tumor tissue in tumor-containing tissue was identified with 100% sensitivity, and the absence of tumor in no-tumor containing tissue was identified with 100% specificity. As gold standard for the determination of the sensitivity and the specificity, we considered the conventional histopathological classification. In summary, shifted-excitation Raman difference spectroscopy could be potentially very useful to support histopathological diagnosis in breast pathology.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Beckmann, Matthias Prof. Dr.
Lehrstuhl für Geburtshilfe und Frauenheilkunde
Bräuer, Andreas PD Dr.
Erlangen Graduate School in Advanced Optical Technologies
Erber, Ramona Dr. med.
Pathologisches Institut
Fasching, Peter PD Dr.
Professur für Translationale Frauenheilkunde und Geburtshilfe
Gebrekidan, Medhanie
Lehrstuhl für Technische Thermodynamik
Hartmann, Arndt Prof. Dr. med.
Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie


Zitierweisen

APA:
Gebrekidan, M., Erber, R., Hartmann, A., Fasching, P., Emons, J., Beckmann, M., & Bräuer, A. (2018). Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS). Technology in Cancer Research & Treatment, 17. https://dx.doi.org/10.1177/1533033818782532

MLA:
Gebrekidan, Medhanie, et al. "Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS)." Technology in Cancer Research & Treatment 17 (2018).

BibTeX: 

Zuletzt aktualisiert 2019-20-07 um 07:16