Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS)

Gebrekidan M, Erber R, Hartmann A, Fasching P, Emons J, Beckmann M, Bräuer A (2018)


Publication Type: Journal article

Publication year: 2018

Journal

Book Volume: 17

DOI: 10.1177/1533033818782532

Abstract

We used a shifted-excitation Raman difference spectroscopy method for the ex vivo classification of resected and formalin-fixed breast tissue samples as normal (healthy) tissue, fibroadenoma, or invasive carcinoma. We analyzed 8 tissue samples containing invasive carcinoma that were surrounded by normal tissue and 3 tissue samples with fibroadenoma only. We made various measurement sites on various tissue samples, in total 240 measurements for each type of tissue. Although the acquired raw spectra contain enough information to clearly differentiate between normal and tumor (fibroadenoma and invasive carcinoma) tissue, the differentiation between fibroadenoma and invasive carcinoma was possible only after the shifted-excitation Raman difference spectroscopy isolation of pure Raman spectra from the heavily fluorescence interfered raw spectra. We used 784 and 785 nm as excitation wavelengths for the shifted-excitation Raman difference spectroscopy method. The differences in the obtained pure Raman spectra are assigned to the different chemical compositions of normal breast tissue, fibroadenoma, and invasive breast carcinoma. Principal component analysis and linear discriminant analysis showed excellent classification results in the Raman shift range between 1000 and 1800 cm-1. Invasive breast carcinoma was identified with 99.15% sensitivity, and the absence of invasive carcinoma was identified with 90.40% specificity. Tumor tissue in tumor-containing tissue was identified with 100% sensitivity, and the absence of tumor in no-tumor containing tissue was identified with 100% specificity. As gold standard for the determination of the sensitivity and the specificity, we considered the conventional histopathological classification. In summary, shifted-excitation Raman difference spectroscopy could be potentially very useful to support histopathological diagnosis in breast pathology.

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How to cite

APA:

Gebrekidan, M., Erber, R., Hartmann, A., Fasching, P., Emons, J., Beckmann, M., & Bräuer, A. (2018). Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS). Technology in Cancer Research & Treatment, 17. https://dx.doi.org/10.1177/1533033818782532

MLA:

Gebrekidan, Medhanie, et al. "Breast Tumor Analysis Using Shifted-Excitation Raman Difference Spectroscopy (SERDS)." Technology in Cancer Research & Treatment 17 (2018).

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