Sellar Region Atypical Teratoid/Rhabdoid Tumors (ATRT) in Adults Display DNA Methylation Profiles of the ATRT-MYC Subgroup

Johann PD, Bens S, Oyen F, Wagener R, Giannini C, Perry A, Raisanen JM, Reis GF, Nobusawa S, Arita K, Felsberg J, Reifenberger G, Agaimy A, Buslei R, Capper D, Pfister SM, Schneppenheim R, Siebert R, Fruehwald MC, Paulus W, Kool M, Hasselblatt M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

American Journal of Surgical Pathology Lippincott, Williams & Wilkins

Book Volume: 42

Pages Range: 506-511

Journal Issue: 4

DOI: 10.1097/PAS.0000000000001023

Abstract

Atypical teratoid/rhabdoid tumor (ATRT) is a highly malignant brain tumor predominantly encountered in infants. Mutations of the SMARCB1 gene are the characteristic genetic lesion. A small group of ATRT stands out clinically, because these tumors are located in the sellar region of adults. To investigate if sellar region ATRT in adults represents a molecular distinct entity, we characterized molecular alterations in 7 sellar region ATRTs in adults as compared with 150 pediatric ATRTs and 47 pituitary adenomas using SMARCB1 sequencing, multiplex ligation-dependent probe amplification and fluorescence in situ hybridization as well as DNA methylation profiling. The median age of the 6 female and 1 male patients was 56 years. On histopathologic examination, all tumors were malignant rhabdoid tumors showing loss of SMARCB1/INI1 protein expression. Two cases displayed compound heterozygous SMARCB1 point mutations, 3 cases showed heterozygous SMARCB1 deletions with point mutations of the other allele and 1 case a homozygous SMARCB1 deletion; in 1 case, underlying SMARCB1 alterations could not be identified. On unsupervised hierarchical cluster analysis of DNA methylation profiles, sellar region ATRTs did not form a distinct group, but clustered with ATRT-MYC, 1 of 3 recently described molecular subgroups of ATRT. On analysis of DNA methylation array intensity data, only 1 sellar region ATRT showed characteristic features of pediatric ATRT-MYC, that is, major copy number losses affecting the SMARCB1 region. In conclusion, these results suggest that sellar region ATRTs in adults form a clinically distinct entity with a different mutational spectrum, but epigenetic similarities with pediatric ATRTs of the ATRT-MYC subgroup.

Authors with CRIS profile

Abbas Agaimy Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie

Involved external institutions

Nationales Centrum für Tumorerkrankungen (NCT) Heidelberg DE Germany (DE) Universität Ulm DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Mayo Clinic US United States (USA) (US) University of California San Francisco (UCSF) US United States (USA) (US) University of Texas Southwestern Medical Center (UT Southwestern) US United States (USA) (US) Memorial Regional Hospital US United States (USA) (US) Gunma University JP Japan (JP) Kagoshima University JP Japan (JP) Heinrich-Heine-Universität Düsseldorf DE Germany (DE) Sozialstiftung Bamberg DE Germany (DE) Charité - Universitätsmedizin Berlin DE Germany (DE) Klinikum Augsburg DE Germany (DE) Universitätsklinikum Münster DE Germany (DE)

How to cite

APA:

Johann, P.D., Bens, S., Oyen, F., Wagener, R., Giannini, C., Perry, A.,... Hasselblatt, M. (2018). Sellar Region Atypical Teratoid/Rhabdoid Tumors (ATRT) in Adults Display DNA Methylation Profiles of the ATRT-MYC Subgroup. American Journal of Surgical Pathology, 42(4), 506-511. https://dx.doi.org/10.1097/PAS.0000000000001023

MLA:

Johann, Pascal D., et al. "Sellar Region Atypical Teratoid/Rhabdoid Tumors (ATRT) in Adults Display DNA Methylation Profiles of the ATRT-MYC Subgroup." American Journal of Surgical Pathology 42.4 (2018): 506-511.

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