MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma

Dietrich P, Kuphal S, Spruss T, Hellerbrand C, Boßerhoff AK (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Pigment Cell & Melanoma Research Wiley-Blackwell

DOI: 10.1111/pcmr.12698

Abstract

The network of molecular players is similar when comparing neural crest-derived, actively migrating melanoblasts to melanoma cells. However, melanoblasts are sensitive to differentiation-initiating signals at their target site (epidermis), while melanoma cells maintain migratory and undifferentiated features. We aimed at identifying downregulated genes in melanoma that are particularly upregulated in melanoblasts. Loss of such genes could contribute to stabilization of a dedifferentiated, malignant phenotype in melanoma. We determined that microRNA-622 (miR-622) expression was strongly downregulated in melanoma cells and tissues compared to melanocytes and melanoblast-related cells. miR-622 expression correlated with survival of patients with melanoma. miR-622 re-expression inhibited clonogenicity, proliferation, and migration in melanoma. Inhibition of miR-622 in melanocytes induced enhanced migration. Kirsten rat sarcoma (KRAS) was identified as a major functional target of miR-622 in melanoma. We conclude that miR-622 is a novel tumor suppressor in melanoma and identify the miR-622-KRAS axis as potential therapeutic target.

Authors with CRIS profile

Peter Dietrich Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Silke Kuphal Lehrstuhl für Biochemie und Molekulare Medizin Claus Hellerbrand Professur für Biochemie und Molekulare Pathobiologie Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Additional Organisation(s)

Emil-Fischer-Zentrum (Emil Fischer Center) Interdisziplinäres Zentrum für Klinische Forschung

Involved external institutions

Universität Regensburg DE Germany (DE)

How to cite

APA:

Dietrich, P., Kuphal, S., Spruss, T., Hellerbrand, C., & Boßerhoff, A.K. (2018). MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma. Pigment Cell & Melanoma Research. https://dx.doi.org/10.1111/pcmr.12698

MLA:

Dietrich, Peter, et al. "MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma." Pigment Cell & Melanoma Research (2018).

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