Measuring Lymphocyte Proliferation in Response to Specific Antigen and Mitogen Stimuli Using Flow Cytometry
Heider S, Strobel J, Mempel W, Eckstein R, Weisbach VG (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 62
Pages Range: 1857-1878
Journal Issue: 10
DOI: 10.7754/Clin.Lab.2016.160106
Abstract
BACKGROUND: We present a multivariate test system using flow cytometry after in-vitro lymphocyte stimulation using 5 mitogens and 7 antigens to describe in-vitro immunofunction. METHODS: The present work is a crucial step towards establishing a simple, CFSE-based, multivariate test system that can describe the dynamics of stimulus-induced lymphocyte proliferation with considerably more precision than is possible with the radionucleotide method using 3H-thymidine. Using multicolour flow cytometry, our method allows additional phenotyping of the proliferating cells and quantifies the proliferation behaviour by precisely resolving daughter generations and determining the precursor frequency. CONCLUSIONS: Taking the calculated apoptosis parameters into account not only provides additional information about the stimulus-specific response behaviour but also improves the validity of the commonly used proliferation indices. Not only can we confirm previous findings that healthy people have marked differences in a multivariate test system in terms of the individual in-vitro reactivity to various stimuli but also substantiate that the response pattern of an individual is remarkably constant. In follow-up studies we can show for the first time that the results of immunofunctional testing do not change over a period of at least 6 months and appear to be an inherent characteristic of the individual and thus possibly have a genetic basis.
Authors with CRIS profile
Joachim Strobel
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie
Reinhold Eckstein
Department of Transfusion Medicine and Haemostaseology
Volker Günter Weisbach
Medizinische Fakultät
Involved external institutions
Germany (DE)How to cite
APA:
Heider, S., Strobel, J., Mempel, W., Eckstein, R., & Weisbach, V.G. (2016). Measuring Lymphocyte Proliferation in Response to Specific Antigen and Mitogen Stimuli Using Flow Cytometry. Clinical Laboratory, 62(10), 1857-1878. https://dx.doi.org/10.7754/Clin.Lab.2016.160106
MLA:
Heider, Stefan, et al. "Measuring Lymphocyte Proliferation in Response to Specific Antigen and Mitogen Stimuli Using Flow Cytometry." Clinical Laboratory 62.10 (2016): 1857-1878.
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