Surface Features of Recombinant Spider Silk Protein eADF4(kappa 16)-Made Materials are Well-Suited for Cardiac Tissue Engineering

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Petzold J, Aigner TB, Touska F, Zimmermann K, Scheibel T, Engel F
Zeitschrift: Advanced Functional Materials
Jahr der Veröffentlichung: 2017
Band: 27
Heftnummer: 36
ISSN: 1616-301X
eISSN: 1616-3018


Abstract

Cardiovascular diseases causing high morbidity and mortality represent a major socioeconomic burden. The primary cause of impaired heart function is often the loss of cardiomyocytes. Thus, novel therapies aim at restoring the lost myocardial tissue. One promising approach is cardiac tissue engineering. Previously, it is shown that Antheraea mylitta silk protein fibroin is a suitable material for cardiac tissue engineering, however, its quality is difficult to control. To overcome this limitation, the interaction of primary rat heart cells with engineered Araneus diadematus fibroin 4 (kappa 16) (eADF4(kappa 16)) is investigated here, which is engineered based on the sequence of ADF4 by replacing the glutamic acid residue in the repetitive unit of its core domain with lysine. The data demonstrate that cardiomyocytes, fibroblasts, endothelial cells, and smooth muscle cells attach well to eADF4(kappa 16) films on glass coverslips which provide an engineered surface with a polycationic character. Moreover, eADF4(kappa 16) films have, in contrast to fibronectin films, no hypertrophic effect but allow the induction of cardiomyocyte hypertrophy. Finally, cardiomyocytes grown on eADF4(kappa 16) films respond to pro-proliferative factors and exhibit proper cell-to-cell communication and electric coupling. Collectively, these data demonstrate that designed recombinant eADF4(kappa 16)-based materials are promising materials for cardiac tissue engineering.


FAU-Autoren / FAU-Herausgeber

Engel, Felix Prof. Dr. rer. nat.
Professur für Experimentelle Nieren- und Kreislaufforschung
Zimmermann, Katharina Prof. Dr. med.
Heisenbergprofessur für Experimentelle Schmerzforschung


Zusätzliche Organisationseinheit(en)
Muscle Research Center Erlangen (MURCE)


Autor(en) der externen Einrichtung(en)
Universität Bayreuth


Zitierweisen

APA:
Petzold, J., Aigner, T.B., Touska, F., Zimmermann, K., Scheibel, T., & Engel, F. (2017). Surface Features of Recombinant Spider Silk Protein eADF4(kappa 16)-Made Materials are Well-Suited for Cardiac Tissue Engineering. Advanced Functional Materials, 27(36).

MLA:
Petzold, Jana, et al. "Surface Features of Recombinant Spider Silk Protein eADF4(kappa 16)-Made Materials are Well-Suited for Cardiac Tissue Engineering." Advanced Functional Materials 27.36 (2017).

BibTeX: 

Zuletzt aktualisiert 2018-21-11 um 10:08