Comparison of Simultaneous CD34+ and CD3+ Quantification with a Modified Stem Cell Enumeration Kit on Two Different Flow Cytometers

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Details zur Publikation

Autor(en): Strobel J, Hauck-Dlimi B, Weisbach VG, Eckstein R, Zingsem J, Strasser E
Zeitschrift: Clinical Laboratory
Jahr der Veröffentlichung: 2016
Band: 62
Heftnummer: 11
Seitenbereich: 2213-2218
ISSN: 1433-6510


Abstract

BACKGROUND: Quantification of CD34+ cells in peripheral blood stem cell apheresis is normally performed by single platform flow cytometric measurements according to the ISHAGE protocol. Peripheral blood stem cell concentrates (PBSC) produced by apheresis normally contain many T cells. Those T cells can be used for production of donor lymphocyte infusion doses, if abundant amounts of CD34+ cells have been collected. Therefore, it is of interest to know both the CD3+ and the CD34+ cell count of allogeneic PBSC. This is the first study comparing the performance of a modified ISHAGE protocol allowing additional quantification of CD3+ cells on two different flow cytometers, the FACSCalibur and the FACSVerse, respectively.
METHODS: CD45+ and CD34+ cell concentrations were measured using a standard and a modified ISHAGE protocol including CD3+ cell quantification on both machines. All cell concentrations were measured using a Trucount bead based stem cell enumeration kit. The FACSVerse machine can additionally be equipped with a sample volume sensor allowing cell quantification without using beads. The samples analysed were taken from granulocyte-colony-stimulating factor mobilized peripheral blood stem cell apheresis procedures (pre- and post-apheresis, and apheresis concentrate).
RESULTS: There were no significant differences in cell concentrations measured by the standard and modified ISHAGE protocol, regardless of which machine had been used when using bead quantification. No significant differences between the results of the two flow cytometers using the modified ISHAGE protocol were observed. Pearson´s correlation was always > 0.96, and regression coefficients were higher than 0.93. The only significant differences were observed between bead quantification and volume sensor quantification on the FACSVerse machine.
CONCLUSIONS: The modified ISHAGE protocol can effectively be used on both flow cytometers tested, especially if bead quantification is used.


FAU-Autoren / FAU-Herausgeber

Eckstein, Reinhold Prof. Dr. med.
Transfusionsmedizinische und Hämostaseologische Abteilung in der Chirurgischen Klinik
Hauck-Dlimi, Barbara PD Dr.
Medizinische Fakultät
Strasser, Erwin Prof. Dr.
Medizinische Fakultät
Strobel, Joachim
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie
Weisbach, Volker Günter Prof. Dr.
Medizinische Fakultät
Zingsem, Jürgen Prof. Dr.
Medizinische Fakultät


Zitierweisen

APA:
Strobel, J., Hauck-Dlimi, B., Weisbach, V.G., Eckstein, R., Zingsem, J., & Strasser, E. (2016). Comparison of Simultaneous CD34+ and CD3+ Quantification with a Modified Stem Cell Enumeration Kit on Two Different Flow Cytometers. Clinical Laboratory, 62(11), 2213-2218. https://dx.doi.org/10.7754/Clin.Lab.2016.160429

MLA:
Strobel, Joachim, et al. "Comparison of Simultaneous CD34+ and CD3+ Quantification with a Modified Stem Cell Enumeration Kit on Two Different Flow Cytometers." Clinical Laboratory 62.11 (2016): 2213-2218.

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Zuletzt aktualisiert 2018-07-11 um 17:08