Impact of collection programs for the generation of monocyte apheresis products on product quality and composition as starting material for the generation of cellular therapeutics

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Pfeiffer H, Völkl S, Gary R, Mackensen A, Achenbach S, Strasser E, Aigner M
Zeitschrift: Transfusion
Jahr der Veröffentlichung: 2018
Band: 58
Heftnummer: 9
Seitenbereich: 2175-2183
ISSN: 0041-1132


Abstract

BACKGROUND: With the discontinuation of the last generation of apheresis machines, new options for monocyte apheresis became available. As apheresis products play a crucial role in the generation of new cellular therapeutics (e.g., generation of dendritic cells [DCs] or precursor for T-cell experiments) we sought to compare two different collection programs for potential benefits or disadvantages due to different composition of the cellular products.
STUDY DESIGN AND METHODS: Composition of discontinuously and continuously collected monocyte products from the same 13 donors was analyzed. For further evaluation as starting material for manufacturing of cellular therapeutics typically used steps such as Ficoll Isopaque, cryoconservation and monocyte isolation, with subsequent generation of mature DCs (mDCs) and assessment of T-cell function, were performed on seven of these apheresis pairs.
RESULTS: Yield of total cells, monocytes, and mDCs was equal with both methods. T-cell composition did not differ significantly in content of CD3+, CD4+, and CD8+ cells. Differentiation status and cytokine production of CD8+ T cells upon stimulation with cytomegalovirus pp65 antigen was not significantly different.
CONCLUSION: Both methods seem comparably suited for the generation of cellular products. If the intended use is "fresh" (without cryoconservation), continuously harvested cells show better cell numbers, while discontinuously harvested cells show better recovery after cryoconservation.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Achenbach, Susanne Dr. med.
Transfusionsmedizinische und Hämostaseologische Abteilung in der Chirurgischen Klinik
Mackensen, Andreas Prof. Dr.
Lehrstuhl für Innere Medizin V
Pfeiffer, Hella Dr.
Medizinische Fakultät
Strasser, Erwin Prof. Dr.
Medizinische Fakultät
Völkl, Simon Dr. rer. nat.
Medizinische Klinik 5 - Hämatologie und Internistische Onkologie


Zitierweisen

APA:
Pfeiffer, H., Völkl, S., Gary, R., Mackensen, A., Achenbach, S., Strasser, E., & Aigner, M. (2018). Impact of collection programs for the generation of monocyte apheresis products on product quality and composition as starting material for the generation of cellular therapeutics. Transfusion, 58(9), 2175-2183. https://dx.doi.org/10.1111/trf.14817

MLA:
Pfeiffer, Hella, et al. "Impact of collection programs for the generation of monocyte apheresis products on product quality and composition as starting material for the generation of cellular therapeutics." Transfusion 58.9 (2018): 2175-2183.

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Zuletzt aktualisiert 2018-20-11 um 12:53