A model of chronic enthesitis and new bone formation characterized by multimodal imaging

Czegley C, Gillmann C, Schauer C, Seyler L, Reinwald C, Hahn M, Uder M, Jochmann K, Naschberger E, Stock M, Schett G, Bäuerle T, Hoffmann M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Disease Models & Mechanisms Company of Biologists: OAJ / Company of Biologists

DOI: 10.1242/dmm.034041

Abstract

Enthesitis is a key feature of several different rheumatic diseases. Its pathophysiology is only partially known due to the lack of access to human tissue and the shortage of solid animal models for enthesitis. Here we aimed to develop a model, which mimics the effector phase of enthesitis and reliably leads to inflammation and new bone formation.Enthesitis was induced by local injection of monosodium urate crystals (MSU) into the metatarsal entheses of wild type (WT) or oxidative burst-deficient Ncf1** mice. Quantitative variables of inflammation (edema, swelling) and vascularization (tissue perfusion) were assessed by MRI, bone forming-activity by [18F]-fluoride-PET and destruction of cortical bone and new bone formation by CT. Non-invasive imaging was validated by histochemical and histomorphometric analysis.While injection of MSU crystals into WT mice triggered transient mild enthesitis with no new bone formation, Ncf1** mice developed chronic enthesitis accompanied by massive enthesiophytes. In MRI, inflammation and blood flow in the entheses were chronically increased while PET/CT showed osteoproliferation with enthesiophyte formation. Histochemical analyses showed chronic inflammation, increased vascularization, osteoclast differentiation and bone deposition in the affected entheseal sites.Herein we describe a fast and reliable effector model of chronic enthesitis, which is characterized by a combination of inflammation, vascularization and new bone formation. This model will help to disentangle the molecular pathways involved in the effector phase of enthesitis.

Authors with CRIS profile

Clarissa Gillmann Institute of Radiology Christiane Reinwald Department of Medicine 3 – Rheumatology and Immunology Michael Uder Lehrstuhl für Diagnostische Radiologie Elisabeth Naschberger Medizinische Fakultät Michael Stock Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Georg Schett Lehrstuhl für Innere Medizin III Tobias Bäuerle Professur für Multimodale Bildgebung in der präklinischen Forschung Markus Hoffmann Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

Universität Duisburg-Essen (UDE) DE Germany (DE)

How to cite

APA:

Czegley, C., Gillmann, C., Schauer, C., Seyler, L., Reinwald, C., Hahn, M.,... Hoffmann, M. (2018). A model of chronic enthesitis and new bone formation characterized by multimodal imaging. Disease Models & Mechanisms. https://doi.org/10.1242/dmm.034041

MLA:

Czegley, Christine, et al. "A model of chronic enthesitis and new bone formation characterized by multimodal imaging." Disease Models & Mechanisms (2018).

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