Cleaved N-terminal histone tails distinguish between NADPH oxidase (NOX)-dependent and NOX-independent pathways of neutrophil extracellular trap formation

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Details zur Publikation

Autor(en): Pieterse E, Rother N, Yanginlar C, Gerretsen J, Boeltz S, Munoz LE, Herrmann M, Pickkers P, Hilbrands LB, Van Der Vlag J
Zeitschrift: Annals of the Rheumatic Diseases
Jahr der Veröffentlichung: 2018
ISSN: 0003-4967


Abstract

OBJECTIVES: Neutrophil extracellular traps (NETs) act in various rheumatic diseases. Although NET formation was originally described as a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)-dependent pathway, it appears that there are also NOX-independent pathways of NET release. Currently, no tools are available that can discriminate between both NET-forming pathways. We aimed to develop a serological method allowing the discrimination between NETs generated through NOX-dependent or NOX-independent pathways.
METHODS: Histones from in vitro generated NOX-dependent and NOX-independent NETs were characterised with a panel of lupus-derived antibodies against N-terminal histone tails using immunofluorescence microscopy, western blot and ELISA. NETs in patients with NET-associated diseases, that is, rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), psoriatic arthritis (PsA) and sepsis, were characterised in sandwich ELISAs employing antibodies against myeloperoxidase (MPO) and N-terminal histone tails as detecting and capturing antibodies, respectively. Functional responses of endothelial cells to NOX-dependent and NOX-independent NETs were assessed as well.
RESULTS: Neutrophil elastase cleaves the N-terminal tails of core histones during NOX-dependent, but not during NOX-independent NET formation. Consequently, the detection of MPO-histone complexes with antibodies against N-terminal histone tails allows discrimination between NETs formed through a NOX-dependent or NOX-independent manner. Characterisation of in vivo circulating NETs revealed the presence of NOX-independent NETs in RA, SLE and sepsis, but NOX-dependent NETs in PsA. NOX-independent NETs displayed an increased capacity to activate endothelial cells when compared with NOX-dependent NETs.
CONCLUSIONS: These results indicate heterogeneity in NET-forming pathways in vivo and highlight the need for disease-specific strategies to prevent NET-mediated pathology.


FAU-Autoren / FAU-Herausgeber

Herrmann, Martin Prof. Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie


Autor(en) der externen Einrichtung(en)
Radboud university medical center / Radboudumc of voluit Radboud Universitair Medisch Centrum (UMC)


Zitierweisen

APA:
Pieterse, E., Rother, N., Yanginlar, C., Gerretsen, J., Boeltz, S., Munoz, L.E.,... Van Der Vlag, J. (2018). Cleaved N-terminal histone tails distinguish between NADPH oxidase (NOX)-dependent and NOX-independent pathways of neutrophil extracellular trap formation. Annals of the Rheumatic Diseases. https://dx.doi.org/10.1136/annrheumdis-2018-213223

MLA:
Pieterse, Elmar, et al. "Cleaved N-terminal histone tails distinguish between NADPH oxidase (NOX)-dependent and NOX-independent pathways of neutrophil extracellular trap formation." Annals of the Rheumatic Diseases (2018).

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Zuletzt aktualisiert 2018-05-11 um 15:23