Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury

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Details zur Publikation

Autor(en): Pyzik M, Rath T, Kuo TT, Win S, Baker K, Hubbard JJ, Grenha R, Gandhi A, Kraemer TD, Mezo AR, Taylor ZS, Mcdonnell K, Nienaber V, Andersen JT, Mizoguchi A, Blumberg L, Purohit S, Jones SD, Christianson G, Lencer WI, Sandlie I, Kaplowitz N, Roopenian DC, Blumberg RS
Zeitschrift: Proceedings of the National Academy of Sciences
Jahr der Veröffentlichung: 2017
Band: 114
Heftnummer: 14
Seitenbereich: E2862-E2871
ISSN: 0027-8424
eISSN: 1091-6490


Abstract

The neonatal crystallizable fragment receptor (FcRn) is responsible for maintaining the long half-life and high levels of the two most abundant circulating proteins, albumin and IgG. In the latter case, the protective mechanism derives from FcRn binding to IgG in the weakly acidic environment contained within endosomes of hematopoietic and parenchymal cells, whereupon IgG is diverted from degradation in lysosomes and is recycled. The cellular location and mechanism by which FcRn protects albumin are partially understood. Here we demonstrate that mice with global or liver-specific FcRn deletion exhibit hypoalbuminemia, albumin loss into the bile, and increased albumin levels in the hepatocyte. In vitro models with polarized cells illustrate that FcRn mediates basal recycling and bidirectional transcytosis of albumin and uniquely determines the physiologic release of newly synthesized albumin into the basal milieu. These properties allow hepatic FcRn to mediate albumin delivery and maintenance in the circulation, but they also enhance sensitivity to the albumin-bound hepatotoxin, acetaminophen (APAP). As such, global or liver-specific deletion of FcRn results in resistance to APAP-induced liver injury through increased albumin loss into the bile and increased intracellular albumin scavenging of reactive oxygen species. Further, protection from injury is achieved by pharmacologic blockade of FcRn-albumin interactions with monoclonal antibodies or peptide mimetics, which cause hypoalbuminemia, biliary loss of albumin, and increased intracellular accumulation of albumin in the hepatocyte. Together, these studies demonstrate that the main function of hepatic FcRn is to direct albumin into the circulation, thereby also increasing hepatocyte sensitivity to toxicity.


Zusätzliche Organisationseinheit(en)
Medizinische Klinik 1 - Gastroenterologie, Pneumologie und Endokrinologie


Autor(en) der externen Einrichtung(en)
Biogen Hemophilia Inc.
Boston Children's Hospital
Brigham and Women's Hospital (BWH)
Howard Hughes Medical Institute
Massachusetts General Hospital
Syntimmune
University of Oslo
University of Southern California (USC)
Zenobia Therapeutics Inc


Zitierweisen

APA:
Pyzik, M., Rath, T., Kuo, T.T., Win, S., Baker, K., Hubbard, J.J.,... Blumberg, R.S. (2017). Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. Proceedings of the National Academy of Sciences, 114(14), E2862-E2871. https://dx.doi.org/10.1073/pnas.1618291114

MLA:
Pyzik, Michal, et al. "Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury." Proceedings of the National Academy of Sciences 114.14 (2017): E2862-E2871.

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Zuletzt aktualisiert 2018-26-10 um 14:31