Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution

Shpacovitch V, Sidorenko I, Lenssen JE, Temchura V, Weichert F, Muller H, Überla K, Zybin A, Schramm A, Hergenroder R (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Sensors MDPI

Book Volume: 17

Journal Issue: 2

DOI: 10.3390/s17020244

Abstract

The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles-microvesicles (100-1000 nm)-also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our findings expand analytical features and the application field of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.

Authors with CRIS profile

Vladimir Temchura Institute of Clinical and Molecular Virology Klaus Überla Institute of Clinical and Molecular Virology

Involved external institutions

Leibniz-Institut für Analytische Wissenschaften / Leibniz Institute for Analytical Sciences (ISAS) DE Germany (DE) MIVITEC GmbH DE Germany (DE) Technische Universität Dortmund DE Germany (DE) Universitätsklinikum Essen DE Germany (DE)

How to cite

APA:

Shpacovitch, V., Sidorenko, I., Lenssen, J.E., Temchura, V., Weichert, F., Muller, H.,... Hergenroder, R. (2017). Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution. Sensors, 17(2). https://dx.doi.org/10.3390/s17020244

MLA:

Shpacovitch, Victoria, et al. "Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution." Sensors 17.2 (2017).

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