ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones

Journal article


Publication Details

Author(s): Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Sudhof TC, Rhee JS, Brose N
Journal: Journal of Cell Biology
Publication year: 2017
Volume: 216
Journal issue: 4
Pages range: 1143-1161
ISSN: 0021-9525


Abstract

Presynaptic active zones (AZs) are unique subcellular structures at neuronal synapses, which contain a network of specific proteins that control synaptic vesicle (SV) tethering, priming, and fusion. Munc13s are core AZ proteins with an essential function in SV priming. In hippocampal neurons, two different Munc13s-Munc13-1 and bMunc13-2-mediate opposite forms of presynaptic short-term plasticity and thus differentially affect neuronal network characteristics. We found that most presynapses of cortical and hippocampal neurons contain only Munc13-1, whereas ∼10% contain both Munc13-1 and bMunc13-2. Whereas the presynaptic recruitment and activation of Munc13-1 depends on Rab3-interacting proteins (RIMs), we demonstrate here that bMunc13-2 is recruited to synapses by the AZ protein ELKS1, but not ELKS2, and that this recruitment determines basal SV priming and short-term plasticity. Thus, synapse-specific interactions of different Munc13 isoforms with ELKS1 or RIMs are key determinants of the molecular and functional heterogeneity of presynaptic AZs.


Additional Organisation
Psychiatrische und Psychotherapeutische Klinik


External institutions with authors

Charité - Universitätsmedizin Berlin
Georg-August-Universität Göttingen
Houston Texas Medical Center
Max-Planck-Institut für biophysikalische Chemie (Karl-Friedrich-Bonhoeffer-Institut) / Max Planck Institute for Biophysical Chemistry
Max-Planck-Institut für Experimentelle Medizin
Max-Planck-Institut für Neurobiologie / Max Planck Institute of Neurobiology
Stanford University
University of Texas Southwestern Medical Center (UT Southwestern)


How to cite

APA:
Kawabe, H., Mitkovski, M., Kaeser, P.S., Hirrlinger, J., Opazo, F., Nestvogel, D.,... Brose, N. (2017). ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones. Journal of Cell Biology, 216(4), 1143-1161. https://dx.doi.org/10.1083/jcb.201606086

MLA:
Kawabe, Hiroshi, et al. "ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones." Journal of Cell Biology 216.4 (2017): 1143-1161.

BibTeX: 

Last updated on 2018-22-10 at 12:47