CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion

Journal article


Publication Details

Author(s): Heger L, Balk S, Lühr J, Heidkamp GF, Lehmann C, Hatscher L, Purbojo A, Hartmann A, Garcia-Martin F, Nishimura SI, Cesnjevar R, Nimmerjahn F, Dudziak D
Journal: Frontiers in Immunology
Publication year: 2018
Volume: 9
ISSN: 1664-3224


Abstract

Dendritic cells (DCs) are major players for the induction of immune responses. Apart from plasmacytoid DCs (pDCs), human DCs can be categorized into two types of conventional DCs: CD141+ DCs (cDC1) and CD1c+ DCs (cDC2). Defining uniquely expressed surface markers on human immune cells is not only important for the identification of DC subpopulations but also a prerequisite for harnessing the DC subset-specific potential in immunomodulatory approaches, such as antibody-mediated antigen targeting. Although others identified CLEC9A as a specific endocytic receptor for CD141+ DCs, such a receptor for CD1c+ DCs has not been discovered, yet. By performing transcriptomic and flow cytometric analyses on human DC subpopulations from different lymphohematopoietic tissues, we identified CLEC10A (CD301, macrophage galactose-type C-type lectin) as a specific marker for human CD1c+ DCs. We further demonstrate that CLEC10A rapidly internalizes into human CD1c+ DCs upon binding of a monoclonal antibody directed against CLEC10A. The binding of a CLEC10A-specific bivalent ligand (the MUC-1 peptide glycosylated with N-acetylgalactosamine) is limited to CD1c+ DCs and enhances the cytokine secretion (namely TNFα, IL-8, and IL-10) induced by TLR 7/8 stimulation. Thus, CLEC10A represents not only a candidate to better define CD1c+ DCs-due to its high endocytic potential-CLEC10A also exhibits an interesting candidate receptor for future antigen-targeting approaches.


FAU Authors / FAU Editors

Cesnjevar, Robert Prof. Dr.
Kinderherzchirurgische Abteilung in der Herzchirurgischen Klinik
Dudziak, Diana Prof. Dr.
Professur für die Biologie Dendritischer Zellen
Hartmann, Arndt Prof. Dr. med.
Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie
Heger, Lukas Dr.
Hautklinik
Heidkamp, Gordon Frederik
Hautklinik
Lehmann, Christian Dr. rer. nat.
Hautklinik
Lühr, Jennifer
Frauenklinik
Nimmerjahn, Falk Prof. Dr.
Lehrstuhl für Genetik
Purbojo, Ariawan Dr. med.
Kinderherzchirurgische Abteilung in der Herzchirurgischen Klinik


External institutions with authors

Hokkaido University (Hokudai) / 北海道大学


How to cite

APA:
Heger, L., Balk, S., Lühr, J., Heidkamp, G.F., Lehmann, C., Hatscher, L.,... Dudziak, D. (2018). CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion. Frontiers in Immunology, 9. https://dx.doi.org/10.3389/fimmu.2018.00744

MLA:
Heger, Lukas, et al. "CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion." Frontiers in Immunology 9 (2018).

BibTeX: 

Last updated on 2019-20-07 at 07:11