Bone Tissue Engineering Under Xenogeneic-Free Conditions in a Large Animal Model as a Basis for Early Clinical Applicability

Weigand A, Beier J, Schmid R, Knorr T, Kilian D, Goetzl R, Gerber T, Horch RE, Boos A (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Tissue Engineering: Parts A, B, and C Mary Ann Liebert

Book Volume: 23

Pages Range: 208-222

Journal Issue: 5-6

DOI: 10.1089/ten.TEA.2016.0176

Abstract

For decades, researchers have been developing a range of promising strategies in bone tissue engineering with the aim of producing a significant clinical benefit over existing therapies. However, a major problem concerns the traditional use of xenogeneic substances for the expansion of cells, which complicates direct clinical transfer. The study's aim was to establish a totally autologous sheep model as a basis for further preclinical studies and future clinical application. Ovine mesenchymal stromal cells (MSC) were cultivated in different concentrations (0%, 2%, 5%, 10%, and 25%) of either autologous serum (AS) or fetal calf serum (FCS). With an increase of serum concentration, enhanced metabolic activity and proliferation could be observed. There were minor differences between MSC cultivated in AS or FCS, comparing gene and protein expression of osteogenic and stem cell markers, morphology, and osteogenic differentiation. MSC implanted subcutaneously in the sheep model, together with a nanostructured bone substitute, either in stable block or moldable putty form, induced similar vascularization and remodeling of the bone substitute irrespective of cultivation of MSC in AS or FCS and osteogenic differentiation. The bone substitute in block form together with MSC proved particularly advantageous in the induction of ectopic bone formation compared to the cell-free control and putty form. It could be demonstrated that AS is suitable for replacement of FCS for cultivation of ovine MSC for bone tissue engineering purposes. Substantial progress has been made in the development of a strictly xenogeneic-free preclinical animal model to bring future clinical application of bone tissue engineering strategies within reach.

Authors with CRIS profile

Annika Kengelbach-Weigand Department of Plastic and Hand Surgery Rafael Schmid Lehrstuhl für Biochemie und Molekulare Medizin Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Anja Boos Department of Plastic and Hand Surgery

Involved external institutions

Universität Rostock DE Germany (DE)

How to cite

APA:

Weigand, A., Beier, J., Schmid, R., Knorr, T., Kilian, D., Goetzl, R.,... Boos, A. (2017). Bone Tissue Engineering Under Xenogeneic-Free Conditions in a Large Animal Model as a Basis for Early Clinical Applicability. Tissue Engineering: Parts A, B, and C, 23(5-6), 208-222. https://dx.doi.org/10.1089/ten.TEA.2016.0176

MLA:

Weigand, Annika, et al. "Bone Tissue Engineering Under Xenogeneic-Free Conditions in a Large Animal Model as a Basis for Early Clinical Applicability." Tissue Engineering: Parts A, B, and C 23.5-6 (2017): 208-222.

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