Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering

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Details zur Publikation

Autor(en): Vielreicher M, Kralisch D, Völkl S, Sternal F, Arkudas A, Friedrich O
Zeitschrift: Scientific Reports
Jahr der Veröffentlichung: 2018
Band: 8
Heftnummer: 1
ISSN: 2045-2322


Abstract

Biomimetic scaffolds are of great interest to tissue engineering (TE) and tissue repair as they support important cell functions. Scaffold coating with soluble collagen-I has been used to achieve better tissue integration in orthopaedy, however, as collagen persistence was only temporary such efforts were limited. Adequate coverage with cell-derived ECM collagen-I would promise great success, in particular for TE of mechanically challenged tissues. Here, we have used label-free, non-invasive multiphoton microscopy (MPM) to characterise bacterial nanocellulose (BNC) - a promising biomaterial for bone TE - and their potency to stimulate collagen-I formation by mesenchymal stem cells (MSCs). BNC fleeces were investigated by Second Harmonic Generation (SHG) imaging and by their characteristic autofluorescence (AF) pattern, here described for the first time. Seeded MSCs adhered fast, tight and very stable, grew to multilayers and formed characteristic, wide-spread and long-lasting collagen-I. MSCs used micron-sized lacunae and cracks on the BNC surface as cell niches. Detailed analysis using a collagen-I specific binding protein revealed a highly ordered collagen network structure at the cell-material interface. In addition, we have evidence that BNC is able to stimulate MSCs towards osteogenic differentiation. These findings offer new options for the development of engineered tissue constructs based on BNC.


FAU-Autoren / FAU-Herausgeber

Arkudas, Andreas Prof. Dr.
Medizinische Fakultät
Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie
Vielreicher, Martin Dr.
Lehrstuhl für Medizinische Biotechnologie
Völkl, Simon Dr. rer. nat.
Medizinische Klinik 5 - Hämatologie und Internistische Onkologie


Autor(en) der externen Einrichtung(en)
Friedrich-Schiller-Universität Jena


Zitierweisen

APA:
Vielreicher, M., Kralisch, D., Völkl, S., Sternal, F., Arkudas, A., & Friedrich, O. (2018). Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering. Scientific Reports, 8(1). https://dx.doi.org/10.1038/s41598-018-27760-z

MLA:
Vielreicher, Martin, et al. "Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering." Scientific Reports 8.1 (2018).

BibTeX: 

Zuletzt aktualisiert 2019-28-02 um 06:12