Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

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Details zur Publikation

Autorinnen und Autoren: Poller J, Zaloga J, Schreiber E, Unterweger H, Janko C, Radon P, Eberbeck D, Trahms L, Alexiou C, Friedrich RP
Zeitschrift: International Journal of Nanomedicine
Jahr der Veröffentlichung: 2017
Band: 12
Seitenbereich: 3207-3220
ISSN: 1176-9114
eISSN: 1178-2013


Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPIONDex) and lauric acid-coated SPIONs (SPIONLA) with an additional protein corona formed by human serum albumin (SPIONLA-HSA) resulted in very moderate particle uptake and low cytotoxicity, whereas SPIONLAhad in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Poller, Johanna
Professur für Nanomedizin
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


Zusätzliche Organisationseinheit(en)
Exzellenz-Cluster Engineering of Advanced Materials


Einrichtungen weiterer Autorinnen und Autoren

Physikalisch-Technische Bundesanstalt (PTB)


Forschungsbereiche

Nanosicherheit
Exzellenz-Cluster Engineering of Advanced Materials


Zitierweisen

APA:
Poller, J., Zaloga, J., Schreiber, E., Unterweger, H., Janko, C., Radon, P.,... Friedrich, R.P. (2017). Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake. International Journal of Nanomedicine, 12, 3207-3220. https://dx.doi.org/10.2147/IJN.S132369

MLA:
Poller, Johanna, et al. "Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake." International Journal of Nanomedicine 12 (2017): 3207-3220.

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Zuletzt aktualisiert 2018-16-11 um 04:08