Does formalin fixation influence MSCT/CBCT accuracy?

Journal article

Publication Details

Author(s): Seidel A, Bergauer B, Lell M, Buder T, von Wilmowsky C, Dach E, Wichmann M, Matta RE
Journal: Surgical and Radiologic Anatomy
Publication year: 2017
ISSN: 0930-1038


PURPOSE: Advanced imaging modalities, such as multi-slice computed tomography (MSCT) and cone beam computed tomography (CBCT), greatly facilitate diagnostic medicine. In radiological research, it is important to know how accurately a scanned object is visualized, and whether the methodology leads to image distortion. The objective of this study was to evaluate whether formalin fixation impacted the accuracy of virtual 3D bone models generated via CBCT and MSCT using a software-based evaluation method that excluded human measurement errors.
METHODS: A head specimen, with and without formalin preservation, was subjected to MSCT and CBCT scans using the manufacturers' predefined scanning protocols. Digital models of the lower jaw were constructed and superimposed with a master model generated based on optical scanning with an industrial non-contact scanner. Means and standard deviations were calculated to assess accuracy, and a t test was used for comparisons between the fixed and unfixed specimens.
RESULTS: The extent of discrepancy between the fixed and unfixed specimens was analyzed using a total of 200 points (n = 200) in each specimen state. The mean deviation between states was 0.01 mm for MSCT (at both 80 and 140 kV). Mean values from CBCT at 0.4 voxel did not differ between states.
CONCLUSIONS: Our results suggest that formalin fixation of an anatomical specimen does not substantially affect the accuracy of a three-dimensional image generated with CBCT and MSCT. Thus, fixed specimen can be used in future investigations of 3D models without concerns regarding the accuracy.

FAU Authors / FAU Editors

Bergauer, Bastian
Zahnklinik 2 - Zahnärztliche Prothetik
Buder, Thomas Dr.
Lehrstuhl für Anatomie und Zellbiologie
Seidel, Anna Dr.
Zahnklinik 2 - Zahnärztliche Prothetik
von Wilmowsky, Cornelius PD Dr.
Mund-, Kiefer- und Gesichtschirurgische Klinik
Wichmann, Manfred Prof. Dr.
Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere zahnärztliche Prothetik

External institutions with authors

Paracelsus Medizinische Privatuniversität

How to cite

Seidel, A., Bergauer, B., Lell, M., Buder, T., von Wilmowsky, C., Dach, E.,... Matta, R.-E. (2017). Does formalin fixation influence MSCT/CBCT accuracy? Surgical and Radiologic Anatomy.

Seidel, Anna, et al. "Does formalin fixation influence MSCT/CBCT accuracy?" Surgical and Radiologic Anatomy (2017).


Last updated on 2018-08-10 at 16:38