Poly-GP in cerebrospinal fluid linksC9orf72-associated dipeptide repeat expression to the asymptomatic phase of ALS/FTD

Lehmer C, Oeckl P, Weishaupt JH, Volk AE, Diehl-Schmid J, Schroeter ML, Lauer M, Kornhuber J, Levin J, Fassbender K, Landwehrmeyer B, Schludi MH, Arzberger T, Kremmer E, Flatley A, Feederle R, Steinacker P, Weydt P, Ludolph AC, Edbauer D, Otto M (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Embo Molecular Medicine Wiley-VCH Verlag / Wiley Open Access

Book Volume: 9

Pages Range: 859-868

Journal Issue: 7

DOI: 10.15252/emmm.201607486

Abstract

TheC9orf72GGGGCC repeat expansion is a major cause of amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD). Non-conventional repeat translation results in five dipeptide repeat proteins (DPRs), but their clinical utility, overall significance, and temporal course in the pathogenesis of c9ALS/FTD are unclear, although animal models support a gain-of-function mechanism. Here, we established a poly-GP immunoassay from cerebrospinal fluid (CSF) to identify and characterizeC9orf72patients. Significant poly-GP levels were already detectable in asymptomaticC9orf72mutation carriers compared to healthy controls and patients with other neurodegenerative diseases. The poly-GP levels in asymptomatic carriers were similar to symptomatic c9ALS/FTD cases. Poly-GP levels were not correlated with disease onset, clinical scores, and CSF levels of neurofilaments as a marker for axonal damage. Poly-GP determination in CSF revealed aC9orf72mutation carrier in our cohort and may thus be used as a diagnostic marker in addition to genetic testing to screen patients. Presymptomatic expression of poly-GP and likely other DPR species may contribute to disease onset and thus represents an alluring therapeutic target.

Authors with CRIS profile

Johannes Kornhuber Lehrstuhl für Psychiatrie und Psychotherapie

Involved external institutions

Universität des Saarlandes (UdS) DE Germany (DE) Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) DE Germany (DE) Universitätsklinikum Ulm DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Universitätsklinikum Leipzig DE Germany (DE) Universitätsklinikum Würzburg DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE)

How to cite

APA:

Lehmer, C., Oeckl, P., Weishaupt, J.H., Volk, A.E., Diehl-Schmid, J., Schroeter, M.L.,... Otto, M. (2017). Poly-GP in cerebrospinal fluid linksC9orf72-associated dipeptide repeat expression to the asymptomatic phase of ALS/FTD. Embo Molecular Medicine, 9(7), 859-868. https://doi.org/10.15252/emmm.201607486

MLA:

Lehmer, Carina, et al. "Poly-GP in cerebrospinal fluid linksC9orf72-associated dipeptide repeat expression to the asymptomatic phase of ALS/FTD." Embo Molecular Medicine 9.7 (2017): 859-868.

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